Lorberbaum David S, Gottlieb David
Department of Anatomy and Neurobiology, Washington University School of Medicine, Missouri, USA.
Genesis. 2011 Feb;49(2):66-74. doi: 10.1002/dvg.20696. Epub 2011 Jan 17.
Discovery and characterization of gene promoters, enhancers and repressor binding elements is an important research area in neuroscience. Here, the suitability of embryonic stem cells and their neural derivatives as a model system for this research is investigated. Three neural transgenic constructs (from the Mnx1, Fabp7, and tuba1a genes) that have been validated in transgenic mice were inserted into embryonic stem cells as stable transgenes. These transgenic embryonic stem cells were differentiated into neural cultures and the pattern of transgene expression across a series of inducing conditions determined. The pattern of expression matched that predicted from transgenic mouse experiments for each of the three transgenes. The results show that embryonic stem cells and their neural derivatives comprise a promising model for investigating the mechanisms that control cell- and temporal-specific neural gene transcription.
基因启动子、增强子和阻遏物结合元件的发现与表征是神经科学中的一个重要研究领域。在此，研究了胚胎干细胞及其神经衍生物作为该研究模型系统的适用性。将已在转基因小鼠中得到验证的三种神经转基因构建体（来自Mnx1、Fabp7和tuba1a基因）作为稳定转基因插入胚胎干细胞。这些转基因胚胎干细胞分化为神经培养物，并确定了在一系列诱导条件下转基因表达的模式。表达模式与针对这三种转基因中的每一种从转基因小鼠实验预测的模式相匹配。结果表明，胚胎干细胞及其神经衍生物构成了一个有前景的模型，可用于研究控制细胞和时间特异性神经基因转录的机制。
