Hou Yun, Li Ling, Hu Ming, Jiang Guang-Yu, Wang Qian, Qian Dong-Meng, Yan Zhi-Yong, Zhao Wei, Song Xu-Xia, Wang Bin
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, China.
Bing Du Xue Bao. 2010 Nov;26(6):477-82.
Nerve growth factor (NGF) is mainly secreted by the neuroglia cells, which can exert biological effect through its receptors on the specific target cell surface. NGF is closely related to neurocyte growth, differentiation and apoptosis. As a neurotropic virus, HSV-1 an easily lead to neurocyte, neuroglia cells death or apoptosis. In this study, the U251 human glioma cells were chosen as target cells to study the change of NGF and its receptors in the apoptosis process of HSV-1 infection. Our results showed that U251 cells were permissive to HSV-1 replication. In the apoptosis process of HSV-1 infected U251 cells, the expression of both NGF and P75NTR increased and then decreased, while the expression of TrkA decreased gradually. These result indicated that HSV-1 was able to induce the abnormal expression of NGF and its receptors in U251 cells.
神经生长因子（NGF）主要由神经胶质细胞分泌，它可通过其在特定靶细胞表面的受体发挥生物学效应。NGF与神经细胞的生长、分化及凋亡密切相关。作为一种嗜神经病毒，单纯疱疹病毒1型（HSV-1）易导致神经细胞、神经胶质细胞死亡或凋亡。在本研究中，选用人U251胶质瘤细胞作为靶细胞，研究HSV-1感染凋亡过程中NGF及其受体的变化。我们的结果显示，U251细胞允许HSV-1复制。在HSV-1感染U251细胞的凋亡过程中，NGF和P75NTR的表达均先升高后降低，而TrkA的表达则逐渐降低。这些结果表明，HSV-1能够诱导U251细胞中NGF及其受体的异常表达。
