Department of Microbiology, Qingdao Medical College, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, Shandong, China.
Biomed Environ Sci. 2009 Aug;22(4):354-8. doi: 10.1016/S0895-3988(09)60068-4.
To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV).
U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV.
The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P < 0.05).
HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
探讨人巨细胞病毒（HCMV）感染人脑胶质瘤细胞后内源性神经生长因子（NGF）表达的变化。
体外培养 U251 细胞，用 HCMV AD169 株感染建立病毒感染细胞模型。倒置显微镜下观察 HCMV 感染前后 U251 细胞的形态学变化。用 RT-PCR 和 Western blot 法检测 HCMV 感染前后细胞 NGF 基因和蛋白的表达。
HCMV 感染后第 5 天出现细胞病变效应，而差异 NGF 表达则在第 7 天明显。与对照组相比，感染后第 7 天 U251 细胞 NGF 表达明显降低（P<0.05）。
HCMV 可下调人胶质瘤细胞系 U251 内源性 NGF 水平。
