Centro de Investigaciones Biológicas del Noroeste, S.C. Mar Bermejo No. 195, Colonia Playa Palo de Santa Rita, Código Postal 23090, La Paz, Baja California Sur, Mexico.
J Invertebr Pathol. 2011 May;107(1):65-8. doi: 10.1016/j.jip.2011.02.002. Epub 2011 Feb 21.
The antiviral effect of vp28 or vp26 double-stranded (ds) RNA upon single or consecutive white spot syndrome virus (WSSV) intramuscular challenges with a high infectious dose was evaluated. The vp28 dsRNA showed the highest protection both in single (LT(50)=145h at 10d and 98h at 20d post treatment [dpt]) or consecutive (LT(50)=765h) WSSV challenges compared to vp26 dsRNA (LT(50)=126h at 10 d and 57h at 20dpt vs. consecutive challenge LT(50)=751h). Single WSSV challenges showed that animals treated with vp28 or vp26 dsRNA gradually lost the antiviral effect as virus challenge occurred at 10dpt (cumulative mortality 63% vs. 80%, respectively) or 20dpt (87% vs. 100%, respectively). In contrast, animals treated with vp28 or vp26 dsRNA and consecutively challenged with WSSV showed and extended lower susceptibility to WSSV. All dead animals were WSSV-positive by one-step PCR, whereas all surviving shrimp from single or continuous challenges were WSSV-negative as determined by reverse transcription (RT)-PCR. In conclusion, shrimp treated with a single administration of vp28 or vp26 dsRNA and consecutively challenged with WSSV showed a stronger and longer antiviral response than shrimp exposed once to WSSV at 10 or 20dpt.
评估了 vp28 或 vp26 双链 (ds) RNA 对单次或连续 WSSV 肌肉内挑战（高感染剂量）的抗病毒效果。与 vp26 dsRNA 相比，vp28 dsRNA 在单次（10d 时 LT(50)=145h，20d 时 LT(50)=98h）或连续（10d 时 LT(50)=765h）WSSV 挑战中均表现出最高的保护作用（10d 时 LT(50)=126h，20d 时 LT(50)=57h）。单次 WSSV 挑战表明，在 10dpt（cumulative mortality 63% vs. 80%）或 20dpt（87% vs. 100%）进行病毒挑战时，用 vp28 或 vp26 dsRNA 处理的动物逐渐失去抗病毒作用。相比之下，用 vp28 或 vp26 dsRNA 处理并连续受到 WSSV 挑战的动物对 WSSV 的敏感性降低。所有死亡动物的一步法 PCR 均为 WSSV 阳性，而单次或连续挑战的所有幸存虾均为 RT-PCR 阴性。总之，单次给予 vp28 或 vp26 dsRNA 并连续受到 WSSV 挑战的虾比在 10 或 20dpt 单次接触 WSSV 的虾表现出更强、更长的抗病毒反应。
