一种针对尿路感染中尿路致病性大肠杆菌的方法。
An approach to uropathogenic Escherichia coli in urinary tract infections.
作者信息
Ranjan K Prabhat, Ranjan Neelima, Chakraborty Arindam, Arora D R
机构信息
Department of Microbiology, Pt. B D Sharma Postgraduate Institute of Medical Sciences, Rohtak, Haryana, India.
出版信息
J Lab Physicians. 2010 Jul;2(2):70-3. doi: 10.4103/0974-2727.72152.
PURPOSE
To study the occurrence and characterization of Uropathogenic Escherichia Coli (UPEC) in cases with urinary tract infections.
MATERIALS AND METHODS
A total of 220 symptomatic cases from urinary tract infections and 50 stool samples from apparently healthy individuals were included. The colonies identified as Escherichia Coli were screened for virulence factors, that is, hemolysin, Mannose Resistant and Mannose Sensitive Hemagglutination (MRHA, MSHA), Cell surface hydrophobicity, and Serum resistance.
RESULTS
Among the 220 cases 91 (41.36%) were hemolytic, 68 (30.90%) showed MRHA, 58 (26.36%) were cell surface hydrophobicity positive, and 72 (32.72%) were serum-resistant. In 50 controls, three (6%) were hemolytic, six (12%) showed MRHA, nine (18%) showed cell surface hydrophobicity, and 12 (24%) were serum-resistant. The difference between cases and controls for hemolysis and MRHA were significant (P<0.001 and P<0.01, respectively). A total of 14 atypical E. coli were isolated from the urine and all showed the presence of one or the other virulence markers. Out of the 18 mucoid E.coli isolated, 10 were serum-resistant.
CONCLUSIONS
The present study revealed that out of 220 urinary isolates, 151 could be labeled as UPEC.
目的
研究尿路感染病例中尿路致病性大肠杆菌（UPEC）的发生情况及特征。
材料与方法
纳入220例有症状的尿路感染病例以及50例表面健康个体的粪便样本。对鉴定为大肠杆菌的菌落进行毒力因子筛查，即溶血素、抗甘露糖和对甘露糖敏感的血凝反应（MRHA、MSHA）、细胞表面疏水性和血清抗性。
结果
在220例病例中，91例（41.36%）溶血，68例（30.90%）表现出MRHA，58例（26.36%）细胞表面疏水性呈阳性，72例（32.72%）具有血清抗性。在50例对照中，3例（6%）溶血，6例（12%）表现出MRHA，9例（18%）表现出细胞表面疏水性，12例（24%）具有血清抗性。病例组与对照组在溶血和MRHA方面的差异具有统计学意义（分别为P<0.001和P<0.01）。从尿液中总共分离出14株非典型大肠杆菌，均显示存在一种或其他毒力标志物。在分离出的18株黏液样大肠杆菌中，10株具有血清抗性。
结论
本研究表明，在220株尿液分离株中，151株可被标记为UPEC。
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