Yamagata N, Oshima H
Department of Biomaterials, Osaka Dental University.
Shika Zairyo Kikai. 1990 Jul;9(4):541-54.
This study evaluated the cytotoxic effects of three common restorative materials on early passage cultured cells derived from human gingiva (HG cells). Other early passage cultured cells derived from human dental pulp tissues (HP cells) and L cells established obtained from mouse subcutaneous tissues, were also examined. Cytotoxicity was assessed according to the uptake of neutral red and dye exclusion with nigrosin. The cytotoxicity was in the order of glass ionomer cement (highest), light cured composite resin and dental amalgam. HG cells were the least sensitive to the materials tested. Both filtration of extracts and addition of serum into the extract affected the cytotoxicity. Glass ionomer cement increased the acidity of the extract, but the two other materials did not. Dissolution of metal ions, aluminium, tin, copper, mercury and zinc occurred from the materials. The extract of the composite resin had a different absorbance. This study disclosed differences in the cellular reaction to the restorative materials. Thus, utilization of early passage cultured cells is necessary for biological evaluation of the dental materials in vitro.
本研究评估了三种常用修复材料对源自人牙龈的原代培养细胞（HG细胞）的细胞毒性作用。还检测了源自人牙髓组织的其他原代培养细胞（HP细胞）以及从小鼠皮下组织获得的L细胞。根据中性红摄取和台盼蓝染料排斥法评估细胞毒性。细胞毒性顺序为玻璃离子水门汀（最高）、光固化复合树脂和牙科汞合金。HG细胞对所测试材料最不敏感。提取物的过滤和向提取物中添加血清均影响细胞毒性。玻璃离子水门汀增加了提取物的酸度，但其他两种材料没有。材料中出现了金属离子铝、锡、铜、汞和锌的溶解。复合树脂提取物具有不同的吸光度。本研究揭示了细胞对修复材料反应的差异。因此，在体外对牙科材料进行生物学评价时，使用原代培养细胞是必要的。
