• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[聚山梨酯80修饰的神经毒素纳米颗粒及其对血脑屏障的转运与细胞毒性]

[Polysorbate-80 modified neurotoxin nanoparticle with its transport and cytotoxicity against blood-brain barrier].

作者信息

Zhao Yan-min, Xia Ai-xiao, Wei Ying-hui, Ruan Ye-ping, Li Fan-zhu

机构信息

College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.

出版信息

Yao Xue Xue Bao. 2010 Oct;45(10):1312-6.

PMID:21348312
Abstract

This study was aimed at the transport across blood-brain barrier (BBB) of polysorbate-80 modified neurotoxin loaded polybutylcyanoacrylate nanoparticle (P-80-NT-NP) and its cytotoxicity. An in vitro model of BBB using rat brain microvascular endothelial cells (rBMECs) was established. The cytotoxicity of P-80-NT-NP was measured by the MTT assays, where neurotoxin (NT), nanoparticle (NP), neurotoxin nanoparticle (NT-NP) as control, and the permeability of P-80-NT-NP was determined by using of Millicell insert coculture with rBMECs and fluorescence spectrophotometry. MTT results showed that NT, NP, NT-NP and P-80-NT-NP were avirulent to rBMECs when the concentration of NT was lower than 200 ng x mL(-1). But the cytotoxicity of NP, NT-NP and P-80-NT-NP would be augmented accordingly as concentration increased (P < 0.01), causing obvious reductions of cell survival rate, with no significant difference between them (P > 0.05). When the concentration of NT was 150 ng x mL(-1), the permeability on rBMECs of P-80-NT-NP and NT-NP were both significantly higher than that of NT (P < 0.01), and the permeability of P-80-NT-NP was greater than that of NT-NP (P < 0.05). In conclusion, polysorbate-80 modified neurotoxin nanoparticles can transport across the BBB, while concentration of NT is greater than 200 ng x mL(-1), P-80-NT-NP has a little cytotoxicity against rBMECs.

摘要

本研究旨在探讨聚山梨酯80修饰的载神经毒素聚氰基丙烯酸正丁酯纳米粒（P-80-NT-NP）的血脑屏障（BBB）转运及其细胞毒性。建立了以大鼠脑微血管内皮细胞（rBMECs）为模型的体外血脑屏障模型。采用MTT法检测P-80-NT-NP的细胞毒性，以神经毒素（NT）、纳米粒（NP）、神经毒素纳米粒（NT-NP）作为对照，通过与rBMECs共培养的Millicell小室和荧光分光光度法测定P-80-NT-NP的通透性。MTT结果显示，当NT浓度低于200 ng x mL(-1)时，NT、NP、NT-NP和P-80-NT-NP对rBMECs均无毒性。但NP、NT-NP和P-80-NT-NP的细胞毒性会随浓度增加而相应增强（P < 0.01），导致细胞存活率明显降低，三者之间差异无统计学意义（P > 0.05）。当NT浓度为150 ng x mL(-1)时，P-80-NT-NP和NT-NP对rBMECs的通透性均显著高于NT（P < 0.01），且P-80-NT-NP的通透性大于NT-NP（P < 0.05）。综上所述，聚山梨酯80修饰的神经毒素纳米粒能够透过血脑屏障，当NT浓度大于200 ng x mL(-1)时，P-80-NT-NP对rBMECs有轻微细胞毒性。

相似文献

1
[Polysorbate-80 modified neurotoxin nanoparticle with its transport and cytotoxicity against blood-brain barrier].[聚山梨酯80修饰的神经毒素纳米颗粒及其对血脑屏障的转运与细胞毒性]
Yao Xue Xue Bao. 2010 Oct;45(10):1312-6.
2
Nanoparticle-mediated delivery of neurotoxin-II to the brain with intranasal administration: an effective strategy to improve antinociceptive activity of neurotoxin.经鼻腔给予神经毒素-II 的纳米颗粒递送至脑内：提高神经毒素的抗伤害活性的有效策略。
Drug Dev Ind Pharm. 2012 Jan;38(1):123-8. doi: 10.3109/03639045.2011.592533. Epub 2011 Jul 1.
3
Cationic albumin conjugated pegylated nanoparticle with its transcytosis ability and little toxicity against blood-brain barrier.具有转胞吞作用且对血脑屏障毒性小的阳离子白蛋白共轭聚乙二醇化纳米颗粒。
Int J Pharm. 2005 May 13;295(1-2):247-60. doi: 10.1016/j.ijpharm.2005.01.043.
4
Transport of Poly(n-butylcyano-acrylate) nanoparticles across the blood-brain barrier in vitro and their influence on barrier integrity.聚（正丁基氰基丙烯酸酯）纳米粒子在体外透过血脑屏障的转运及其对屏障完整性的影响。
Biochem Biophys Res Commun. 2011 Mar 4;406(1):64-9. doi: 10.1016/j.bbrc.2011.01.110. Epub 2011 Feb 3.
5
Transport of stavudine, delavirdine, and saquinavir across the blood-brain barrier by polybutylcyanoacrylate, methylmethacrylate-sulfopropylmethacrylate, and solid lipid nanoparticles.聚氰基丙烯酸丁酯、甲基丙烯酸甲酯-甲基丙烯酸磺丙酯和固体脂质纳米粒介导司他夫定、地拉韦定和沙奎那韦透过血脑屏障的研究
Int J Pharm. 2007 Aug 1;340(1-2):143-52. doi: 10.1016/j.ijpharm.2007.03.012. Epub 2007 Mar 12.
6
Influence of particle size on transport of methotrexate across blood brain barrier by polysorbate 80-coated polybutylcyanoacrylate nanoparticles.粒径对聚山梨酯80包衣聚氰基丙烯酸正丁酯纳米粒载运甲氨蝶呤透过血脑屏障的影响
Int J Pharm. 2006 Mar 9;310(1-2):213-9. doi: 10.1016/j.ijpharm.2005.11.040. Epub 2006 Jan 19.
7
Antinociceptive properties of nasal delivery of neurotoxin-loaded nanoparticles coated with polysorbate-80.聚山梨酯 80 包裹的载神经毒素纳米颗粒经鼻腔给药的抗伤害感受特性。
Peptides. 2011 Jul;32(7):1526-9. doi: 10.1016/j.peptides.2011.05.002. Epub 2011 May 10.
8
Establishment of coculture model of blood-brain barrier in vitro for nanoparticle's transcytosis and toxicity evaluation.建立用于纳米颗粒转胞吞作用及毒性评估的体外血脑屏障共培养模型。
Yao Xue Xue Bao. 2006 Apr;41(4):296-304.
9
Electromagnetic interference in the permeability of saquinavir across the blood-brain barrier using nanoparticulate carriers.使用纳米颗粒载体时，沙奎那韦透过血脑屏障的渗透性中的电磁干扰。
Int J Pharm. 2008 Mar 3;351(1-2):271-81. doi: 10.1016/j.ijpharm.2007.09.020. Epub 2007 Sep 22.
10
The first step into the brain: uptake of NIO-PBCA nanoparticles by endothelial cells in vitro and in vivo, and direct evidence for their blood-brain barrier permeation.进入大脑的第一步：体外和体内内皮细胞对NIO-PBCA纳米颗粒的摄取及其血脑屏障渗透的直接证据。
ChemMedChem. 2008 Sep;3(9):1395-403. doi: 10.1002/cmdc.200800130.

引用本文的文献

1
Assessment of the therapeutic potential of lutein and beta-carotene nanodispersions in a rat model of fibromyalgia.叶黄素和β-胡萝卜素纳米分散体在纤维肌痛大鼠模型中的治疗潜力评估。
Sci Rep. 2023 Nov 12;13(1):19712. doi: 10.1038/s41598-023-46980-6.
2
Brain-Targeted Polysorbate 80-Emulsified Donepezil Drug-Loaded Nanoparticles for Neuroprotection.用于神经保护的脑靶向聚山梨醇酯80乳化载多奈哌齐纳米颗粒
Nanoscale Res Lett. 2021 Aug 18;16(1):132. doi: 10.1186/s11671-021-03584-1.