Goodwin David G, Strobl Jeannine S, Lindsay David S
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061-0342, USA.
J Parasitol. 2011 Feb;97(1):148-51. doi: 10.1645/GE-2536.1. Epub 2010 Sep 14.
An increasing interest in the association of the presence of antibodies to Toxoplasma gondii and the development of schizophrenia in patients has been generated over the last several years. Some antischizophrenia agents have been shown to have activity against T. gondii in cell culture assays and to ameliorate behavioral changes associated with chronic T. gondii infection in rats. In the present study, we examined the effects of commonly used antipsychotic and mood stabilizing agents (haloperidol, clozapine, fluphenazine, trifluoperazine, and thioridazine) for activity against developing tachyzoites of the RH strain of T. gondii in human fibroblast cell cultures. Neither haloperidol nor clozapine had a measurable effect. Fluphenazine had an IC(50) of 1.7 µM, thioridazine had an IC(50) of 1.2 µM, and trifluoperazine had an IC(50) of 3.8 µM. Our study demonstrates that some agents used to treat schizophrenia have the ability to inhibit T. gondii proliferation in cell culture.
在过去几年里，人们对弓形虫抗体的存在与患者精神分裂症发展之间的关联越来越感兴趣。在细胞培养试验中，一些抗精神分裂症药物已被证明对弓形虫具有活性，并能改善与大鼠慢性弓形虫感染相关的行为变化。在本研究中，我们检测了常用的抗精神病药物和心境稳定剂（氟哌啶醇、氯氮平、氟奋乃静、三氟拉嗪和硫利达嗪）对人成纤维细胞培养物中弓形虫RH株速殖子生长的抑制活性。氟哌啶醇和氯氮平均未产生可测量的效果。氟奋乃静的半数抑制浓度（IC50）为1.7μM，硫利达嗪的IC50为1.2μM，三氟拉嗪的IC50为3.8μM。我们的研究表明，一些用于治疗精神分裂症的药物具有在细胞培养中抑制弓形虫增殖的能力。
