Department of Functional Genomics and Proteomics, Faculty of Science, CEITEC, Masaryk University, Brno, Czech Republic.
Curr Protein Pept Sci. 2011 Mar;12(2):112-25. doi: 10.2174/138920311795684913.
Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.
蛋白质磷酸化是一种重要的翻译后修饰方式，可调节大量基因的表达。它参与了许多关键的细胞过程，并且在很大程度上决定了蛋白质组的特征。由于多种生物学和技术原因，蛋白质磷酸化的鉴定需要结合多种不同的方法。本综述特别强调了与通过质谱（MS）确定磷酸化位点的存在及其定位相关的分析策略。还讨论了不同磷酸蛋白质组学研究目标的各种分析方法组合的可行性。
