Department of Medical Informatics and Biomathematics, University of Münster, Münster and Munich Leukemia Laboratory, Munich, Germany.
Bioinformatics. 2011 Apr 15;27(8):1162-3. doi: 10.1093/bioinformatics/btr102. Epub 2011 Feb 23.
The R453Plus1Toolbox is an R/Bioconductor package for the analysis of 454 Sequencing data. Projects generated with Roche's data analysis software can be imported into R allowing advanced and customized analyses within the R/Bioconductor environment for sequencing data. Several methods were implemented extending the current functionality of Roche's software. These extensions include methods for quality assurance and annotation of detected variants. Further, a pipeline for the detection of structural variants, e.g. balanced chromosomal translocations, is provided.
The R453Plus1Toolbox is implemented in R and available at http://www.bioconductor.org/. A vignette outlining typical workflows is included in the package.
Supplementary data are available at Bioinformatics online.
R453Plus1Toolbox 是一个用于分析 454 测序数据的 R/Bioconductor 软件包。Roche 数据分析软件生成的项目可以导入到 R 中，从而在 R/Bioconductor 环境中对测序数据进行高级和定制的分析。该软件包扩展了 Roche 软件的当前功能，实现了多种方法。这些扩展包括用于检测到的变异体的质量保证和注释的方法。此外，还提供了用于检测结构变异体（例如平衡染色体易位）的流水线。
R453Plus1Toolbox 是用 R 实现的，可在 http://www.bioconductor.org/ 获得。该软件包中包含一个概述典型工作流程的简介。
补充数据可在 Bioinformatics 在线获得。
