Pathology and Laboratory Medicine, Mayo Clinic, Rochester, MN 55905, USA.
Am J Clin Pathol. 2011 Mar;135(3):461-7. doi: 10.1309/AJCP4VFKA5FCMZNA.
A multisite study was conducted to assess the performance of the Aperio digital pathology system (Aperio Technologies, Vista, CA) for reading estrogen receptor (ER) and progesterone receptor (PR) slides on a computer monitor. A total of 520 formalin-fixed breast tissue specimens were assayed at 3 clinical sites for ER and PR (260 each). Percentage and average staining intensity of positive nuclei were assessed. At each site, 3 pathologists performed a blinded reading of the glass slides using their microscopes initially and later using digital images on a computer monitor. Comparable percentages of agreements were obtained for manual microscopy (MM) and manual digital slide reading (MDR) (ER, percentage of positive nuclei with cutoffs: MM, 91.3%-99.0%/MDR, 91.3%-100.0%; PR, percentage of positive nuclei with cutoffs: MM, 83.8%-99.0%/MDR, 76.3%-100.0%). Reading ER and PR slides on a computer monitor using the Aperio digital pathology system is equivalent to reading the slides with a conventional light microscope.
一项多中心研究评估了 Aperio 数字病理学系统（Aperio Technologies，Vista，CA）在计算机监视器上读取雌激素受体（ER）和孕激素受体（PR）幻灯片的性能。共有 520 例福尔马林固定的乳腺组织标本在 3 个临床中心进行 ER 和 PR 检测（各 260 例）。评估阳性核的百分比和平均染色强度。在每个中心，3 名病理学家最初使用显微镜进行玻璃载片的盲法读取，然后在计算机监视器上使用数字图像进行读取。手动显微镜（MM）和手动数字载玻片读取（MDR）获得了可比的一致性百分比（ER，有截止值的阳性核百分比：MM，91.3%-99.0%/MDR，91.3%-100.0%；PR，有截止值的阳性核百分比：MM，83.8%-99.0%/MDR，76.3%-100.0%）。使用 Aperio 数字病理学系统在计算机监视器上读取 ER 和 PR 幻灯片等同于使用传统的光学显微镜读取幻灯片。
