Department of Materials Science and Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA.
J Tissue Eng. 2010 Jun 16;2010:209860. doi: 10.4061/2010/209860.
Cell differentiation on glutaraldehyde cross-linked ovalbumin scaffolds was the main focus of this research. Salt leaching and freeze drying were used to create a three-dimensional porous structure. Average pore size was 147.84 ± 40.36 μm and 111.79 ± 30.71 μm for surface and cross sectional area, respectively. Wet compressive strength and elastic modulus were 6.8 ± 3.6 kPa. Average glass transition temperature was 320.1 ± 1.4°C. Scaffolds were sterilized with ethylene oxide prior to seeding MC3T3-E1 cells. Cells were stained with DAPI and Texas red to determine morphology and proliferation. Average cell numbers increased between 4-hour- and 96-hour-cultured scaffolds. Alkaline phosphatase and osteocalcin levels were measured at 3, 7, 14, and 21 days. Differentiation studies showed an increase in osteocalcin at 21 days and alkaline phosphatase levels at 14 days, both indicating differentiation occurred. This work demonstrated the use of ovalbumin scaffolds for a bone tissue engineering application.
本研究的重点是戊二醛交联卵清白蛋白支架上的细胞分化。采用盐析和冷冻干燥的方法制备三维多孔结构。表面和横截面的平均孔径分别为 147.84 ± 40.36 μm 和 111.79 ± 30.71 μm。湿态压缩强度和弹性模量分别为 6.8 ± 3.6 kPa。平均玻璃化转变温度为 320.1 ± 1.4°C。在接种 MC3T3-E1 细胞之前，用环氧乙烷对支架进行灭菌。用 DAPI 和 Texas Red 对细胞进行染色，以确定细胞的形态和增殖情况。在 4 小时和 96 小时培养的支架中，细胞数量平均增加。在第 3、7、14 和 21 天测量碱性磷酸酶和骨钙素水平。分化研究表明，21 天时骨钙素水平增加，14 天时碱性磷酸酶水平增加，这表明发生了分化。这项工作证明了卵清白蛋白支架在骨组织工程应用中的使用。
