Department of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University; Hainan Provincial Stomatology Center, Haikou 570208, China; Qingdao Stomatological Hospital, Qingdao 266000, China.
Department of Oral Implantation, Affiliated Haikou Hospital, Xiangya Medical School, Central South University; Hainan Provincial Stomatology Center, Haikou 570208, China.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110749. doi: 10.1016/j.msec.2020.110749. Epub 2020 Feb 19.
Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration. Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties. In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method. The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively. The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR). In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting. The results revealed that the NPP was successfully incorporated in the C-HA scaffolds. The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold. The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP. Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective. The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes. Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.
骨组织工程利用成骨诱导支架已发展成为一种有前途的骨再生方法。珍珠粉由于其生物活性特征和良好的机械性能，最近在骨再生领域引起了关注。为了在结构和组成上模拟骨组织，本研究通过冷冻干燥法制备了含有纳米珍珠粉（NPP）的壳聚糖-透明质酸（C-HA）支架。通过扫描电子显微镜（SEM）、压汞仪、接触角和万能材料试验机分别研究了支架的微观结构、孔隙率、亲水性和机械性能。通过 X 射线衍射（XRD）测试了 NPP 的晶体形态，通过傅里叶变换红外（FTIR）研究了三种成分之间的相互作用。另一方面，通过细胞计数试剂盒-8（CCK-8）、碱性磷酸酶活性（ALP）、RT-qPCR 和 Western blot 检测了支架的生物相容性、成骨特性和成骨相关基因表达。结果表明，NPP 成功地掺入到 C-HA 支架中。支架的亲水性和机械性能随 NPP 含量的增加而增加，而孔隙形态受到干扰，特别是 25%支架。支架的孔隙率在 89%至 93%之间，随着 NPP 的增加略有降低。细胞培养试验表明，含有较高 NPP 比例的支架有利于 MC3T3-E1 细胞的增殖和分化，其中 10wt%和 25wt%纳米珍珠粉支架效果最佳。分化可能是通过上调 Col αI、OCN、OPN 和 Runx2 基因的表达来促进的。因此，纳米珍珠粉/壳聚糖-透明质酸（NPP/C-HA）支架可能成为一种有前途的骨组织工程生物材料。
