Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE 50.732-970, Brazil.
Curr Microbiol. 2011 May;62(5):1596-601. doi: 10.1007/s00284-011-9903-7. Epub 2011 Feb 25.
The objectives of this study were to determine the distribution of phylogenetic groups among Klebsiella pneumoniae isolates from Recife, Brazil and to assess the relationship between the groups and the isolation sites and resistance profile. Ninety four isolates of K. pneumoniae from hospital or community infections and from normal microbiota were analyzed by gyrA PCR-RFLP, antibiotic susceptibility, and adonitol fermentation. The results revealed the distinction of three phylogenetic groups, as it has also been reported in Europe, showing that these clusters are highly conserved within K. pneumoniae. Group KpI was dominantly represented by hospital and community isolates while groups KpII and KpIII displayed mainly normal microbiota isolates. The resistance to third generation cephalosporins, aztreonam, imipenem, amoxicillin/clavulanic acid, and streptomycin was only observed in KpI. The percentage of resistance was higher in KpI, followed by KpII and KpIII. The differences in the distribution of K. pneumoniae phylogenetic groups observed in this study suggest distinctive clinical and epidemiological characteristics among the three groups, which is important to understand the epidemiology of infections caused by this organism. This is the first study in Brazil on K. pneumoniae isolates from normal microbiota and community infections regarding the distribution of phylogenetic groups based on the gyrA gene.
本研究的目的是确定巴西累西腓市肺炎克雷伯菌分离株的系统发育群分布，并评估这些群与分离部位和耐药谱之间的关系。通过 gyrA PCR-RFLP、抗生素敏感性和山梨醇发酵对 94 株来自医院或社区感染及正常微生物群的肺炎克雷伯菌分离株进行了分析。结果表明，存在三个系统发育群，这与欧洲的报道一致，表明这些群在肺炎克雷伯菌中高度保守。KpI 组主要由医院和社区分离株代表，而 KpII 和 KpIII 组主要显示正常微生物群分离株。仅在 KpI 中观察到对第三代头孢菌素、氨曲南、亚胺培南、阿莫西林/克拉维酸和链霉素的耐药性。KpI 中的耐药率更高，其次是 KpII 和 KpIII。本研究中观察到肺炎克雷伯菌系统发育群分布的差异表明，这三个群具有不同的临床和流行病学特征，这对于理解该病原体引起的感染的流行病学非常重要。这是巴西首次基于 gyrA 基因对来自正常微生物群和社区感染的肺炎克雷伯菌分离株进行系统发育群分布的研究。
