Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Int J Cancer. 2010 Dec 15;127(12):2758-67. doi: 10.1002/ijc.25301.
Although obesity is a risk factor for colorectal cancer, the underlying mechanism is not clear. Adiponectin is an adipokine that binds to 2 types of receptors, AdipoR1 and AdipoR2. The plasma concentrations of adiponectin are reduced in obese individuals and adiponectin has been reported to have anticarcinogenic properties. Furthermore, AdipoR1 and AdipoR2 have been reported to be expressed in several malignancies. However, little is known about the expression of AdipoR1 and AdipoR2 in colorectal cancer and its clinicopathological implications. In addition, the relationship between adiponectin and colorectal cancer has not yet been determined. Here, we sought to investigate adiponectin and adiponectin receptors in relation to colorectal cancer. AdipoR1 and AdipoR2 immunostaining was detected in 72 and 68% of human colorectal cancer tissue, respectively. AdipoR1 and AdipoR2 expression levels were inversely related to T stage. The lowest AdipoR1 and AdipoR2 expression were detected in poorly differentiated adenocarcinoma. RT-PCR also showed the expression of AdipoR1 and AdipoR2 in HCT116 and SW620. MTT assay and TUNEL assay demonstrated the tendency of growth inhibition and apoptosis induction in both cell lines after full-length adiponectin treatment although statistically insignificant. Microarray analysis revealed several gene responses to full-length adiponectin, including upregulation of ENDOGL1 and MT1G. In conclusion, AdipoR1 and AdipoR2 may be intimately related to the progression of colorectal cancer. Further studies may be warranted to assess adiponectin and its receptors as a novel target for inhibition of colorectal cancer growth.
虽然肥胖是结直肠癌的一个风险因素，但潜在的机制尚不清楚。脂联素是一种脂肪细胞因子，与 2 种受体，AdipoR1 和 AdipoR2 结合。肥胖个体的血浆脂联素浓度降低，并且已经报道脂联素有抗癌特性。此外，AdipoR1 和 AdipoR2 已经在几种恶性肿瘤中表达。然而，关于 AdipoR1 和 AdipoR2 在结直肠癌中的表达及其与临床病理的关系知之甚少。此外，脂联素与结直肠癌之间的关系尚未确定。在这里，我们试图研究脂联素和脂联素受体与结直肠癌的关系。在 72%的人结直肠癌组织中检测到 AdipoR1 和 68%的 AdipoR2 免疫染色。AdipoR1 和 AdipoR2 的表达水平与 T 分期呈负相关。在低分化腺癌中检测到最低的 AdipoR1 和 AdipoR2 表达。RT-PCR 还显示 HCT116 和 SW620 中 AdipoR1 和 AdipoR2 的表达。MTT 测定和 TUNEL 测定表明，全长脂联素处理后两种细胞系均有生长抑制和凋亡诱导的趋势，尽管无统计学意义。微阵列分析显示全长脂联素可引起几种基因反应，包括 ENDOGL1 和 MT1G 的上调。总之，AdipoR1 和 AdipoR2 可能与结直肠癌的进展密切相关。可能需要进一步的研究来评估脂联素及其受体作为抑制结直肠癌生长的新靶标。
