Zhang Shuang, Li Hong, Xu Hui, Yang Shi-Jie
Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun 130021, China.
Yao Xue Xue Bao. 2010 Jan;45(1):31-6.
This study is to observe the protection of gross saponins of Tribulus terrestris (GSTT) on cardiocytes impaired by adriamycin (ADR) and approach its mechanism of action. Cardiocytes of neonate rat were cultivated for 72 hours and divided into normal control group, model (ADR 2 mg x L(-1)) group, and GSTT (100, 30, and 10 mg x L(-1)) groups. MTT colorimetric method was deployed to detect cardiocyte survival rate, activities of CK, LDH, AST, SOD, MDA and NO were detected, and apoptosis was detected with flow cytometry. Effect of GSTT on caspase-3 was detected with Western blotting. Compared with control group, contents of CK, LDH, AST, MDA and NO were increased, and activity of SOD was reduced (P < 0.05, P < 0.01, P < 0.001) by ADR. Numbers of survival cells were increased (P < 0.05, P < 0.001), contents of CK, LDH, AST, MDA and NO were decreased, and activity of SOD was increased (P < 0.05, P < 0.01, P < 0.001) by GSTT (100 and 30 mg x L(-1)). Apoptosis of cardiocytes and concentration of caspase-3 can be reduced by GSTT (100 and 30 mg x L(-1)). GSTT can protect cardiocytes impaired by ADR, which are possible involved with its effect of resisting oxygen free radical.
本研究旨在观察刺蒺藜总皂苷（GSTT）对阿霉素（ADR）损伤心肌细胞的保护作用，并探讨其作用机制。将新生大鼠心肌细胞培养72小时，分为正常对照组、模型（ADR 2mg·L⁻¹）组和GSTT（100、30和10mg·L⁻¹）组。采用MTT比色法检测心肌细胞存活率，检测CK、LDH、AST、SOD、MDA和NO的活性，并用流式细胞术检测细胞凋亡。用Western印迹法检测GSTT对caspase-3的影响。与对照组相比，ADR使CK、LDH、AST、MDA和NO含量升高，SOD活性降低（P<0.05，P<0.01，P<0.001）。GSTT（100和30mg·L⁻¹）使存活细胞数增加（P<0.05，P<0.001），CK、LDH、AST、MDA和NO含量降低，SOD活性升高（P<0.05，P<0.01，P<0.001）。GSTT（100和30mg·L⁻¹）可降低心肌细胞凋亡率和caspase-3浓度。GSTT可保护ADR损伤的心肌细胞，其机制可能与其抗氧自由基作用有关。
