Yang Li-Hu, Qiu Jian-Dong, Li Hong-Quan
College of Animal Science and Veterinary Medicine, Agricultural University of Shanxi, Taigu 030801, China.
Yao Xue Xue Bao. 2009 Dec;44(12):1364-70.
Astragalus heteropolysaccharides (AHPS) is obtained from the dried roots of Astragalus membranaceus (Fisch.) Bunge var. mongholious (Bunge) Hsiao. In the present study, we observed its effects on erythrocyte immune adherence function in mice with adjuvant-induced arthritis (AA). The mice were treated intragastrically with AHPS of 1 000, 500, and 250 mg x kg(-1) x d(-1) separately and treated with tripterygium glycosides (TG) of 60 mg x kg(-1) x d(-1) as positive control. The number of complement receptor type 1 (CR1) on erythrocyte, the concentration of circulating immune complex (CIC) in serum and the amount of immune complex (IC) deposition in synovium of knee joint were determined by flow cytometry, polyethylene glycol (PEG-6000) precipitation and ponceau S (P-S) staining and fluorescent immunohistochemistry respectively. The pathological change of knee joint was evaluated by histological section. The results showed that both AHPS and TG improved significantly the primary and secondary local or systemic symptoms of the mice with AA and reduced the synovium hyperplasia, inflammatory cell infiltrate, pannus and cartilage demolish of knee joint, and AHPS of 1 000, 500, and 250 mg x kg(-1) x d(-1) could significantly increase the number of CR1 on erythrocyte, improve the elimination of CIC in the peripheral blood and reduce the deposition of IC in joint synovium in a dose-dependent manner (P < 0.01 or P < 0.05). The results indicate that one of the therapeutic effective mechanisms of AHPS on mice with AA could be to increase gene expression of CR1 of mice with AA.
黄芪杂多糖（AHPS）从蒙古黄芪（Astragalus membranaceus (Fisch.) Bunge var. mongholious (Bunge) Hsiao）的干燥根中提取。在本研究中，我们观察了其对佐剂性关节炎（AA）小鼠红细胞免疫黏附功能的影响。将小鼠分别以1000、500和250 mg·kg⁻¹·d⁻¹的剂量灌胃给予AHPS，并以60 mg·kg⁻¹·d⁻¹的雷公藤多苷（TG）作为阳性对照进行治疗。分别采用流式细胞术、聚乙二醇（PEG - 6000）沉淀法以及丽春红S（P - S）染色和荧光免疫组织化学法测定红细胞上补体受体1（CR1）的数量、血清中循环免疫复合物（CIC）的浓度以及膝关节滑膜中免疫复合物（IC）的沉积量。通过组织学切片评估膝关节的病理变化。结果表明，AHPS和TG均能显著改善AA小鼠的原发性和继发性局部或全身症状，减轻膝关节滑膜增生、炎性细胞浸润、血管翳形成以及软骨破坏，且1000、500和250 mg·kg⁻¹·d⁻¹的AHPS能显著增加红细胞上CR1的数量，改善外周血中CIC的清除，并呈剂量依赖性地减少IC在关节滑膜中的沉积（P < 0.01或P < 0.05）。结果表明，AHPS对AA小鼠的治疗有效机制之一可能是增加AA小鼠CR1的基因表达。
