Li Gang, Li Guang-yan, Ji Hai-Jie, Zhao Wen-jie, Chu Shi-feng, Chen Nai-hong
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Yao Xue Xue Bao. 2010 Aug;45(8):995-1000.
This study is to investigate the influence and the expression of CMTM family of testosterone on spermatogenesis suppression in the male rats treated by gossypol and cyclophosphamide. Gossypol (50 mg kg(-1)) and cyclophosphamide (20 mg kg(-1)) were administered to male rats to induce spermatogenesis suppression. Testosterone propionate was administrated at the dose of 5 mg kg(-1) every other day for 6 times. Sperm was collected from the left caudal epididymis, the count and motility of sperm were analyzed by CASA. Morphological change of testis tissue was observed with HE staining. The expression of CMTM family was examined by Western blotting assay. Gossypol (50 mg kg(-1)) and cyclophosphamide (20 mg kg(-1)) decreased the count and motility of sperm, and the pathological change of testis tissue was also observed. But, testosterone (5 mg kg(-1)) had positive effect. Furthermore, CMTM4 down-expressed remarkably in the gossypol and cyclophosphamide treated rats, the expression of the CMTM4 was up-expressed after testosterone administration. On the contrary, the expression of CMTM2 increased significantly only in gossypol treated male rats, but not in cyclophosphamide treated male rats. The expression of CMTM2 was down-expressed after testosterone administration. However, no obvious change of CMTM2 was observed in cyclophosphamide treated rats. Testosterone did not influence the expression of CKLF1, CMTM3 and CMTM5, the CMTM6, CMTM7 and CMTM8 of CMTM family were not detected in testis tissue. These demonstrated that the spermatogenesis effect of testosterone (5 mg kg(-1)) was associated with the expression of CMTM family, and CMTM2 and CMTM4 may take part in the spermatogenesis process.
本研究旨在探讨CMTM家族在棉酚和环磷酰胺处理的雄性大鼠中对睾酮抑制精子发生的影响及其表达情况。将棉酚（50 mg kg⁻¹）和环磷酰胺（20 mg kg⁻¹）给予雄性大鼠以诱导精子发生抑制。每隔一天以5 mg kg⁻¹的剂量给予丙酸睾酮，共给药6次。从左侧附睾尾收集精子，通过计算机辅助精子分析（CASA）分析精子数量和活力。用苏木精-伊红（HE）染色观察睾丸组织的形态变化。通过蛋白质免疫印迹法检测CMTM家族的表达。棉酚（50 mg kg⁻¹）和环磷酰胺（20 mg kg⁻¹）降低了精子数量和活力，同时也观察到睾丸组织的病理变化。但是，睾酮（5 mg kg⁻¹）具有积极作用。此外，在棉酚和环磷酰胺处理的大鼠中，CMTM4表达明显下调，给予睾酮后CMTM4表达上调。相反，仅在棉酚处理的雄性大鼠中CMTM2表达显著增加，但在环磷酰胺处理的雄性大鼠中未增加。给予睾酮后CMTM2表达下调。然而，在环磷酰胺处理的大鼠中未观察到CMTM2有明显变化。睾酮不影响CKLF1、CMTM3和CMTM5的表达，在睾丸组织中未检测到CMTM家族的CMTM6、CMTM7和CMTM8。这些结果表明，睾酮（5 mg kg⁻¹）对精子发生的作用与CMTM家族的表达有关，CMTM2和CMTM4可能参与精子发生过程。
