Li Ming, Zhang Changqing, Yuan Ting, Chen Shengbao, Lü Ruju
Department of Orthopedics, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233, P.R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011 Jan;25(1):112-6.
To calculate the recovery rate and enrichment factor and to analyse the correlation by measuring the concentrations of platelets, leukocyte, and growth factors in platelet-rich plasma (PRP) so as to evaluate the feasibility and stability of a set of PRP preparation.
The peripheral blood (40 mL) was collected from 30 volunteers accorded with the inclusion criteria, and then 4 mL PRP was prepared using the package produced by Shandong Weigao Group Medical Polymer Company Limited. Automatic hematology analyzer was used to count the concentrations of platelets and leukocyte in whole blood and PRP. The enrichment factor and recovery rate of platelets or leukocyte were calculated; the platelet and leukocyte concentrations of male and female volunteers were measured, respectively. The concentrations of platelet-derived growth factor (PDGF), transforming growth factor beta (TGF-beta), and vascular endothelial growth factor (VEGF) were assayed by ELISA.
The platelet concentrations of whole blood and PRP were (131.40 +/- 29.44) x 10(9)/L and (819.47 +/- 136.32) x 10(9)/L, respectively, showing significant difference (t = 27.020, P = 0.000). The recovery rate of platelets was 60.85% +/- 8.97%, and the enrichment factor was 6.40 +/- 1.06. The leukocyte concentrations of whole blood and PRP were (5.57 +/- 1.91) x 10(12)/L and (32.20 +/- 10.42) x 10(12)/L, respectively, showing significant difference (t = 13.780, P = 0.000). The recovery rate of leukocyte was 58.30% +/- 19.24%, and the enrichment factor was 6.10 +/- 1.93. The concentrations of platelets and leukocyte in PRP were positively correlated with the platelet concentration (r = 0.652, P = 0.000) and leukocyte concentration (r = 0.460, P = 0.011) in whole blood. The concentrations of platelet and leukocyte in PRP between male and female were not significantly different (P > 0.05). The concentrations of PDGF, TGF-beta, and VEGF in PRP were (698.15 +/- 64.48), (681.36 +/- 65.90), and (1071.55 +/- 106.04) ng/mL, which were (5.67 +/- 1.18), (6.99 +/- 0.61), and (5.74 +/- 0.83) times higher than those in the whole blood, respectively. PDGF concentration (r = 0.832, P = 0.020), TGF-beta concentration (r = 0.835, P = 0.019), and VEGF concentration (r = 0.824, P = 0.023) in PRP were positively correlated with platelet concentration of PRP.
PRP with high concentrations of platelets, white blood cells and growth factors can be prepared stably by this package.
通过检测富血小板血浆（PRP）中血小板、白细胞及生长因子的浓度，计算回收率和富集因子，并分析其相关性，以评估一套PRP制备方法的可行性和稳定性。
采集30例符合纳入标准志愿者的外周血（40 mL），采用山东威高集团医用高分子制品股份有限公司生产的套装制备4 mL PRP。使用全自动血液分析仪计数全血及PRP中血小板和白细胞的浓度。计算血小板或白细胞的富集因子和回收率；分别测定男性和女性志愿者的血小板和白细胞浓度。采用酶联免疫吸附测定法（ELISA）检测血小板衍生生长因子（PDGF）、转化生长因子β（TGF-β）和血管内皮生长因子（VEGF）的浓度。
全血和PRP中血小板浓度分别为（131.40±29.44）×10⁹/L和（819.47±136.32）×10⁹/L，差异有统计学意义（t = 27.020，P = 0.000）。血小板回收率为60.85%±8.97%，富集因子为6.40±1.06。全血和PRP中白细胞浓度分别为（5.57±1.91）×10¹²/L和（32.20±10.42）×10¹²/L，差异有统计学意义（t = 13.780，P = 0.000）。白细胞回收率为58.30%±19.24%，富集因子为6.10±1.93。PRP中血小板和白细胞浓度与全血中血小板浓度（r = 0.652，P = 0.000）和白细胞浓度（r = 0.460，P = 0.011）呈正相关。PRP中男性和女性的血小板和白细胞浓度差异无统计学意义（P＞0.05）。PRP中PDGF、TGF-β和VEGF的浓度分别为（698.15±64.48）、（681.36±65.90）和（1071.55±106.04）ng/mL，分别是全血中浓度的（5.67±1.18）、（6.99±0.61）和（5.74±0.83）倍。PRP中PDGF浓度（r = 0.832，P = 0.020）、TGF-β浓度（r = 0.835，P = 0.019）和VEGF浓度（r = 0.824，P = 0.023）与PRP中血小板浓度呈正相关。
使用该套装可稳定制备出含有高浓度血小板、白细胞及生长因子的PRP。
