Everts Peter A M, Brown Mahoney Christine, Hoffmann Johannes J M L, Schönberger Jacques P A M, Box Henk A M, van Zundert André, Knape Johannes T A
Department of Extra Corporeal Blood Management, Catharina Hospital Eindhoven, Eindhoven, The Netherlands.
Growth Factors. 2006 Sep;24(3):165-71. doi: 10.1080/08977190600821327.
In this study, three commercial systems for the preparation of platelet-rich plasma (PRP) were compared and platelet growth factors release was measured.
Ten healthy volunteers donated whole blood that was fractionated by a blood cell separator, and a table-top centrifuge to prepare PRP. Furthermore, an autologous growth factor filter was used to concentrate PRP fractionated by the blood cell separator. PRP was subsequently activated with autologously produced thrombin to degranulate the platelets to measure platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-beta (TGF-beta), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF).
PRP contained significantly higher platelet counts compared with baseline values (p < 0.001). PDGF-AB concentrations were increased more than 18-fold in the platelet gel supernatant when the cell-separator and GPS were used, whereas only a 3-fold increase was seen with the AGF.
The three PRP devices enable the preparation of PRP for the release of high concentrations of platelet growth factor, but showed different harvesting capacities for the collection of concentrated platelets. The administration of thrombin for PRP activation resulted in the release of high concentrations of PDGF-AB and TGF-beta but only when PRP had not been activated during the preparation process in vitro.
在本研究中，比较了三种用于制备富血小板血浆（PRP）的商业系统，并测量了血小板生长因子的释放。
10名健康志愿者捐献全血，通过血细胞分离器和台式离心机进行分离以制备PRP。此外，使用自体生长因子过滤器对通过血细胞分离器分离的PRP进行浓缩。随后用自体产生的凝血酶激活PRP，使血小板脱颗粒，以测量血小板衍生生长因子-AB（PDGF-AB）、转化生长因子-β（TGF-β）、胰岛素样生长因子-1（IGF-1）和血管内皮生长因子（VEGF）。
与基线值相比，PRP中的血小板计数显著更高（p < 0.001）。当使用细胞分离器和GPS时，血小板凝胶上清液中的PDGF-AB浓度增加超过18倍，而使用AGF时仅增加3倍。
这三种PRP装置能够制备用于释放高浓度血小板生长因子的PRP，但在收集浓缩血小板方面显示出不同的采集能力。用于PRP激活的凝血酶给药导致高浓度的PDGF-AB和TGF-β释放，但仅当PRP在体外制备过程中未被激活时。
