[Establishment of embryonic stem cell for evaluation of development neurotoxicity and test the validation of embryonic stem cell].

OBJECTIVE

To establish embryonic stem cell (EST) for evaluation of developmental neurontoxicity and test the validation of EST.

METHODS

RT-PCR method was used to study the influence of fluorouracil (5-FU), diphenylhydantoin (DPH) and Penicillin G at the different concentrations on neuron cells with nestin expression that differentiate from embryonic stem cells (ES). With the results of cells viability (ES and BALB/c) assessed by MTT, the developmental neurotoxicty characteristics of 5-FU, DPH and Penicillin G may be identified clearly. RESULTS; The ID50 D3 nestin concentration of three embryotoxicants were 0.017, 49.4 and 1139 microg/ml. Penicillin G, DPH and 5-FU were discriminated as none-, weak- and strong- developmental neurotoxicity. CONCLUSION; Test compounds among three classes were discriminated correctly. The EST on developmental neurontoxicity could be applied to the safety evaluation in vitro.