[胚胎干细胞试验应用模型的验证研究]
[Study on the validation of application model on embryonic stem test].
作者信息
Yu Zhou, Yan Weixing, Zhang Lianfeng, Xu Haibin
机构信息
Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China.
出版信息
Wei Sheng Yan Jiu. 2008 Sep;37(5):570-3.
OBJECTIVE
According trend of differentiation cardiomyocyte of embryonic stem cell,the model of EST has been built and the validation of the model is needed to be tested. It is profit to improve the method of safety evaluation.
METHODS
The embryonic stem cell differentiates into cardiomyocyte in different concentrations of Penicillin G,DPH and 5-FU with hanging and suspending culture conditions. With the results of cytotoxicity, the different embryonic toxicity characteristics of different substants may be detected clearly.
RESULTS
The ID50 (D3) concentration of three embryo toxicants were 1099, 47.4 and 0.023 microg/ml. Penicillin G, DPH and 5-FU were discriminated as none-embryotoxicity, weak-embryotoxicity and strong-embryotoxicity.
CONCLUSION
Test compounds between three classes were discriminated correctly .The validation of EST model that established by us is high.
目的
根据胚胎干细胞分化为心肌细胞的趋势，建立了胚胎干细胞试验（EST）模型，该模型的有效性有待验证。这有助于改进安全性评价方法。
方法
在不同浓度的青霉素G、双苯并[a,h]蒽（DPH）和5-氟尿嘧啶（5-FU）作用下，采用悬滴悬浮培养条件使胚胎干细胞分化为心肌细胞。根据细胞毒性结果，可以清晰地检测出不同物质的不同胚胎毒性特征。
结果
三种胚胎毒物的半数抑制浓度（ID50，D3）分别为1099、47.4和0.023微克/毫升。青霉素G、DPH和5-FU分别被判定为无胚胎毒性、弱胚胎毒性和强胚胎毒性。
结论
三类受试化合物被正确区分。我们建立的EST模型有效性高。
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