Sekiguchi Biomatrix Signaling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency, Aichi Medical University, Aichi, Japan.
J Dermatol. 2011 Jul;38(7):674-9. doi: 10.1111/j.1346-8138.2010.01050.x. Epub 2010 Oct 6.
The hair follicle is one of the skin appendages that develops through reciprocal epithelial-mesenchymal interactions. Although a large number of studies have been made on the mechanisms of hair follicle development, the whole molecular mechanism that governs hair follicle development remains poorly defined. To further understand the molecular basis of hair follicle development, it is necessary to identify genes that drive hair morphogenesis. As an initial approach, we attempted to identify gene products associated with mouse hair follicle development. Genes upregulated in the vibrissal hair placodes were screened by polymerase chain reaction (PCR)-based cDNA subtraction. The genes thus isolated were evaluated for their hair development-associated induction and spatiotemporal expression by quantitative reverse-transcription-PCR analysis and whole-mount in situ hybridization, respectively. Finally, we identified four genes whose upregulation and spatiotemporal expression in developing hair follicles were confirmed. Successful identification of novel hair development-associated genes will be informative as clues for further characterization of hair follicle development at the molecular level.
毛囊是通过上皮-间充质相互作用发育形成的皮肤附属物之一。尽管已经对毛囊发育的机制进行了大量研究，但控制毛囊发育的整个分子机制仍未完全明确。为了进一步了解毛囊发育的分子基础，有必要鉴定出驱动毛发生成的基因。作为一种初步方法，我们试图鉴定与小鼠毛囊发育相关的基因产物。通过基于聚合酶链反应（PCR）的 cDNA 消减筛选出在触须毛基板中上调的基因。通过定量逆转录-PCR 分析和整体原位杂交分别评估这些分离的基因在毛发发育中的诱导和时空表达。最后，我们鉴定了四个在发育中的毛囊中上调和时空表达得到确认的基因。成功鉴定出与新的毛发生成相关的基因将为进一步在分子水平上阐明毛囊发育提供线索。
