Pongsiri S, Schlegel D, Zimmermann M
Poliklinik für Kieferchirurgie der Universität München.
Dtsch Z Mund Kiefer Gesichtschir. 1990 Sep-Oct;14(5):364-8.
In this study 380 freshly extracted anterior teeth and premolars were stored for periods of 30 to 360 min in different types of media subsequently the residual vitality of periodontal ligament cells was assessed by means of the fluorescein diacetate reaction. It was found that after 90 min of dry storage 88% of the periodontal cells were devitalized, whereas after 180 min of storage in saliva or normal saline solution the loss of vitality was 40% or, respectively, 37%. Stored in Alpha MEM or UHT milk for 360 min 48% or, respectively, 49% of the periodontal ligament cells showed vital reaction.
在本研究中，380颗新鲜拔除的前牙和前磨牙在不同类型的介质中储存30至360分钟，随后通过荧光素二乙酸酯反应评估牙周膜细胞的残余活力。结果发现，干燥储存90分钟后，88%的牙周细胞失去活力，而在唾液或生理盐水中储存180分钟后，活力丧失分别为40%和37%。在α-MEM或超高温灭菌牛奶中储存360分钟后，分别有48%和49%的牙周膜细胞显示出活力反应。
