Technische Universität Berlin, Institut für Chemie, Berlin, Germany.
FEBS J. 2011 May;278(9):1382-90. doi: 10.1111/j.1742-4658.2011.08064.x. Epub 2011 Mar 22.
Most of the biochemical and biophysical processes of proteins take place at membranes, and are thus under the influence of strong local electric fields, which are likely to affect the structure as well as the reaction mechanism and dynamics. To analyse such electric field effects, biomimetic interfaces may be employed that consist of membrane models deposited on nanostructured metal electrodes. For such devices, surface-enhanced resonance Raman and IR absorption spectroscopy are powerful techniques to disentangle the complex interfacial processes of proteins in terms of rotational diffusion, electron transfer, and protein and cofactor structural changes. The present article reviews the results obtained for the haem protein cytochrome c, which is widely used as a model protein for studying the various reaction steps of interfacial redox processes in general. In addition, it is shown that electric field effects may be functional for the natural redox processes of cytochrome c in the respiratory chain, as well as for the switch from the redox to the peroxidase function, one of the key events preceding apoptosis.
大多数蛋白质的生化和生物物理过程都发生在膜上，因此受到强局部电场的影响，这可能会影响蛋白质的结构以及反应机制和动力学。为了分析这种电场效应，可以使用仿生界面，它由沉积在纳米结构金属电极上的膜模型组成。对于此类设备，表面增强共振拉曼和红外吸收光谱是用于分离蛋白质在旋转扩散、电子转移以及蛋白质和辅助因子结构变化方面的复杂界面过程的强大技术。本文综述了广泛用作研究一般界面氧化还原过程中各种反应步骤的模型蛋白的血红素蛋白细胞色素 c 的研究结果。此外，还表明电场效应可能对细胞色素 c 在呼吸链中的自然氧化还原过程以及从氧化还原到过氧化物酶功能的转变（细胞凋亡前的关键事件之一）具有功能。
