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副流感(仙台)病毒蛋白亚基吸附到NIL8仓鼠胚胎细胞后的命运。

The fate of protein subunits of parainfluenza (Sendai) virus after adsorption to NIL8 hamster embryo cells.

作者信息

Kohn A

出版信息

J Gen Virol. 1975 Nov;29(2):179-87. doi: 10.1099/0022-1317-29-2-179.

Abstract

Adsorption of u.v.-inactivated Sendai virus on to NIL8 hamster cells causes fusion of the cells into polykaryocytes within 2 h. "Infected" cells were incubated at 37 degrees C for periods of 10 min to 8 h and their surface proteins iodinated with [125I] catalysed by peroxidase. Structural components of the viral envelope, such as haemagglutin-neuraminidase (HN) and probably also the fusion protein (F) were detected in the cell membrane for periods up to 4 h post infection.

摘要

紫外线灭活的仙台病毒吸附到NIL8仓鼠细胞上会在2小时内使细胞融合成多核细胞。“感染”的细胞在37℃下孵育10分钟至8小时,并用过氧化物酶催化的[125I]对其表面蛋白进行碘化。在感染后长达4小时的时间里,在细胞膜中检测到病毒包膜的结构成分,如血凝素神经氨酸酶(HN),可能还有融合蛋白(F)。

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