从改良安卡拉痘苗病毒中删除 A35 基因可提高免疫原性和同种型转换。
Deletion of the A35 gene from Modified Vaccinia Virus Ankara increases immunogenicity and isotype switching.
机构信息
East Carolina University, Brody School of Medicine, 600 Moye Blvd, 5E106A, Department of Microbiology & Immunology, Greenville, NC 27834, United States.
出版信息
Vaccine. 2011 Apr 12;29(17):3276-83. doi: 10.1016/j.vaccine.2011.02.023. Epub 2011 Feb 23.
Abstract
We show here that the immunogenicity of the Modified Vaccinia Ankara MVA vaccine strain can be improved by deletion of the A35 gene, without diminishing the ability of the virus to replicate. Deletion of the A35 gene resulted in increased virus-specific immunoglobulin production, class switching to IgG isotypes, and virus-specific IFNγ-secreting splenocytes. The MVA35 deletion virus provided excellent protective efficacy against virulent virus challenge. These results suggest that A35 deletion mutant strains will have superior vaccine performance for poxvirus vaccines as well as platform vaccines for other infectious diseases and cancer.
摘要
我们在这里表明，通过删除 A35 基因，可以提高改良安卡拉痘苗病毒（MVA）疫苗株的免疫原性，而不降低病毒的复制能力。删除 A35 基因导致病毒特异性免疫球蛋白产生增加，同种型转换为 IgG 类，并产生病毒特异性 IFNγ 分泌的脾细胞。MVA35 缺失病毒对强毒病毒攻击提供了极好的保护效力。这些结果表明，A35 缺失突变株将在正痘病毒疫苗以及其他传染病和癌症的平台疫苗方面具有更好的疫苗性能。
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