Department of Immunobiology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA.
Virology. 2011 Apr 25;413(1):39-46. doi: 10.1016/j.virol.2011.01.033. Epub 2011 Feb 25.
Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3(+) and CD4(+) naive (CD45RA(+)) and memory (CD45RO(+)) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3(+) and CD4(+) CD45RA(+) and CD45RO(+) T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env(-)R(+) luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.
我们之前的研究表明，与成人血细胞相比，HIV-1 在新生儿（脐带）血单核细胞/巨噬细胞和 T 淋巴细胞中的复制水平更高。然而，目前尚不清楚这种 HIV-1 复制的差异是否也发生在幼稚和/或记忆性 T 淋巴细胞中。因此，我们比较了从五名脐带血和成人血液供体中分离的 CD3(+)和 CD4(+)幼稚（CD45RA(+））和记忆（CD45RO(+））T 淋巴细胞中的 HIV-1 复制。我们发现，与成人血液相比，HIV-1 在从脐带血中分离的 CD3(+)和 CD4(+) CD45RA(+)和 CD45RO(+) T 淋巴细胞中复制水平更高。此外，与成人血细胞相比，脐带血 CD45RA(+)和 CD45RO(+) T 淋巴细胞表面 CD4、CXCR4 和 CCR5 的表达没有差异。此外，我们发现，使用单周期复制有效的 HIV-1-NL4-3-Env(-)R(+)荧光素酶亲嗜性病毒，可增加脐带血 CD45RA(+)和 CD45RO(+) T 淋巴细胞中的 HIV-1 基因表达，该病毒独立于 CD4 和 CXCR4 或 CCR5 表达测量 HIV-1 转录活性。总之，与成人血细胞相比，HIV-1 在脐带血 CD45RA(+)和 CD45RO(+) T 淋巴细胞中的复制水平更高，这种差异复制受 HIV-1 基因表达水平的影响。
