Ohashi T, Arai M, Kato H, Kubo M, Fujii M, Yamamoto N, Iwamoto A, Kannagi M
Department of Immunotherapeutics, Tokyo Medical and Dental University, Japan.
Virology. 1998 May 10;244(2):467-72. doi: 10.1006/viro.1998.9151.
Stromal cell-derived factor 1 (SDF-1) inhibits T-cell tropic (T-tropic) HIV-1 infection in vitro. In this study, we examined the regulatory role of SDF-1 on HIV-1 replication in peripheral blood mononuclear cells (PBMC) of HIV-infected individuals. We found that the amount of SDF-1 mRNA in freshly isolated PBMC of HIV-1 carriers was higher than in healthy donors. Moreover, PBMC from some asymptomatic carriers (ACs) exhibited high levels of SDF-1 mRNA expression. The level of SDF-1 expression in PBMC did not correlate with the magnitude of CD8+ T-cell-mediated suppression of HIV-1 among ACs SDF-1 inhibited HIV-1 replication at the viral entry step, whereas a single-cycle HIV-1 infection system showed that the major part of the CD8+ T-cell-mediated suppression occurs after intracellular penetration of the virus. Our results suggest that SDF-1 acts as a suppressor of virus replication in a CD8+ T-cell-independent mechanism in HIV-infected individuals.
基质细胞衍生因子1（SDF-1）在体外可抑制嗜T细胞型（T-tropic）HIV-1感染。在本研究中，我们检测了SDF-1对HIV感染个体外周血单核细胞（PBMC）中HIV-1复制的调节作用。我们发现，HIV-1携带者新鲜分离的PBMC中SDF-1 mRNA的量高于健康供体。此外，一些无症状携带者（AC）的PBMC表现出高水平的SDF-1 mRNA表达。PBMC中SDF-1的表达水平与AC中CD8 + T细胞介导的HIV-1抑制程度无关。SDF-1在病毒进入步骤抑制HIV-1复制，而单周期HIV-1感染系统显示，CD8 + T细胞介导的抑制作用主要发生在病毒细胞内穿透之后。我们的结果表明，在HIV感染个体中，SDF-1以一种不依赖CD8 + T细胞的机制作为病毒复制的抑制剂。
