Kawakami M
Department of Orthodontics, Osaka University Faculty of Dentistry, Japan.
Osaka Daigaku Shigaku Zasshi. 1990 Jun;35(1):128-46.
The purpose of the present study was to explore the effect of local application with Vitamin D metabolite, 1,25 dihydroxycholecalciferol (1,25 (OH)2D3), on experimental tooth movement in rats. 1. According to Waldo's method, a piece of orthodontic elastic band was inserted between the upper first and second molars of Wistar male rats weighing 200 g. The amount of 20 microliters of 1,25 (OH)2D3(10(-12)-10(-7) M) was injected locally in the submucosal palatal area of the root bifurcation of the right first molar. The left side was injected with vehicle. The number of osteoclasts was counted in a 700 x 1050 micron 2 area of the inter-radicular septum. The number of osteoclasts was dose-dependently increased 2-fold at 10(-10) M 1,25 (OH)2D3 compared to that in the vehicle-injected side. The maximal increase of osteoclast number was observed 3 days after local injection of 10(-10) M 1,25 (OH)2D3 on experimental tooth movement. 2. The upper first molar of Wistar male rats was moved in a buccal direction by a helical spring. The amount of 20 microliters of the local administration of 10(-10) M 1,25 (OH)2D3 was repeated every 3 days until sacrifice at day 20. The tooth movement in the 1,25 (OH)2D3-treated rats was accelerated about 2-fold compared to that in the control rats. 3. The effect of bone formation in the rats receiving experimental tooth movement was examined by fluorescent labeling and quantitative histology. Thus, the local application of 10(-10) M 1,25 (OH)2D3 tended to prevent the decrease of the mineral apposition rate of the alveolar bone following orthodontic tooth movement. 4. Serum samples of these 1,25 (OH)2D3-treated rats was obtained from abdominal aorta 3 hours after the final injection. Serious effects were not found in the values for parameters such as calcium, phosphorus and alkaline phosphatase. These findings suggested that the local use of 1,25 (OH)2D3 on experimental tooth movement in the rats caused increase in osteoclasts number and accelerated tooth movement. However, no obvious side effects were noted. 1,25 (OH)2D3 was expected to stimulate mineral apposition rate of alveolar bone on the tension side.
本研究的目的是探讨局部应用维生素D代谢物1,25 - 二羟胆钙化醇（1,25(OH)₂D₃）对大鼠实验性牙齿移动的影响。1. 根据Waldo法，在体重200 g的雄性Wistar大鼠的上颌第一和第二磨牙之间插入一段正畸橡皮筋。将20微升1,25(OH)₂D₃（10⁻¹² - 10⁻⁷ M）局部注射到右侧第一磨牙牙根分叉处的腭侧黏膜下区域。左侧注射赋形剂。在根间间隔700×1050微米²的区域内计数破骨细胞数量。与注射赋形剂的一侧相比，在10⁻¹⁰ M 1,25(OH)₂D₃时破骨细胞数量呈剂量依赖性增加2倍。在局部注射10⁻¹⁰ M 1,25(OH)₂D₃后3天观察到实验性牙齿移动时破骨细胞数量的最大增加。2. 用螺旋弹簧将雄性Wistar大鼠的上颌第一磨牙向颊侧移动。每3天重复局部给予20微升10⁻¹⁰ M 1,25(OH)₂D₃，直至第20天处死。与对照大鼠相比，1,25(OH)₂D₃处理的大鼠牙齿移动加速约2倍。3. 通过荧光标记和定量组织学检查接受实验性牙齿移动的大鼠的骨形成效果。因此，局部应用10⁻¹⁰ M 1,25(OH)₂D₃倾向于防止正畸牙齿移动后牙槽骨矿物质沉积率的降低。4. 在最后一次注射后3小时从腹主动脉采集这些1,25(OH)₂D₃处理的大鼠的血清样本。钙、磷和碱性磷酸酶等参数的值未发现严重影响。这些发现表明，在大鼠实验性牙齿移动中局部使用1,25(OH)₂D₃会导致破骨细胞数量增加并加速牙齿移动。然而，未观察到明显的副作用。1,25(OH)₂D₃有望刺激张力侧牙槽骨的矿物质沉积率。
