Takano-Yamamoto T, Kawakami M, Yamashiro T
Department of Orthodontics, Osaka University, Faculty of Dentistry, Japan.
J Dent Res. 1992 Aug;71(8):1487-92. doi: 10.1177/00220345920710080501.
The purpose of this study was to compare the amount and rate of tooth movement in young and mature rats administered 1,25(OH)2D3 simultaneous with application of mechanical force. In 30 seven-week-old and 30 28-week-old male Wistar rats, the right maxillary first molar was moved buccally with a fixed appliance. The appliances delivered forces ranging from 5 to 20 g. Twenty microL of 1,25(OH)2D3 (10(-10) and 10(-8) mol/L) was injected locally into the submucosal palatal area of the root bifurcation of the right first molar. The left side was injected with phosphate-buffered saline (PBS). In young rats receiving 10(-10) mol/L 1,25(OH)2D3 every three days, tooth movement significantly increased to 126% of that in PBS-injected control rats on day 20. In 1,25(OH)2D3-injected mature rats, tooth movement was stimulated markedly and increased with 10(-10) mol/L to 245% and with 10(-8) mol/L to 154% of the amount of tooth movement seen in the PBS-injected controls by the end of the experiment. PBS-injected rats had a plateau stage where tooth movement did not occur at all, while there was no such lag-time in the 1,25(OH)2D3-injected group which showed continuous tooth movement. The local injection of 1,25(OH)2D3 did not change serum calcium, phosphate, and alkaline phosphatase activity, and there were no apparent clinical or microscopic side-effects.
本研究的目的是比较在施加机械力的同时给予1,25(OH)₂D₃的年轻和成熟大鼠的牙齿移动量和移动速率。在30只7周龄和30只28周龄的雄性Wistar大鼠中，用固定矫治器将右上颌第一磨牙颊向移动。矫治器施加的力范围为5至20克。将20微升1,25(OH)₂D₃（10⁻¹⁰和10⁻⁸摩尔/升）局部注射到右第一磨牙根分叉处的腭侧粘膜下区域。左侧注射磷酸盐缓冲盐水（PBS）。在每三天接受10⁻¹⁰摩尔/升1,25(OH)₂D₃的年轻大鼠中，在第20天时牙齿移动显著增加至注射PBS的对照大鼠的126%。在注射1,25(OH)₂D₃的成熟大鼠中，牙齿移动受到明显刺激，在实验结束时，10⁻¹⁰摩尔/升组牙齿移动增加至注射PBS对照组的245%，10⁻⁸摩尔/升组增加至154%。注射PBS的大鼠有一个完全没有牙齿移动的平台期，而注射1,25(OH)₂D₃的组没有这样的延迟期，表现为持续的牙齿移动。局部注射1,25(OH)₂D₃未改变血清钙、磷和碱性磷酸酶活性，也没有明显的临床或微观副作用。
