Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Acta Trop. 2011 May;118(2):165-9. doi: 10.1016/j.actatropica.2011.02.009. Epub 2011 Feb 24.
Neurocysticercosis (NCC) caused by T. solium metacestode is an increasingly important health issue in Indian children. The sensitivity and specificity of available serological techniques were low in case of single cysticercus granuloma cases which is a more common feature in Indian patients who are children. Serum samples were collected from 13 clinically and radiologically suggestive NCC children and seropositive by ELISA, 25 clinically and radiologically suggestive NCC children and seronegative by ELISA and 25 control subjects. The 10-30 kDa antigens of T. solium metacestode were subjected to 2-dimensional gel electrophoresis (2D-PAGE) followed by enzyme-linked immunoelectrotransfer blot (EITB) assay to detect antibody in serum. Analysis of 10-30 kDa antigenic fraction 2D-PAGE map showed 31 proteins between 10 and ≤28 kDa and innumerable proteins between >28 and 30 kDa with the Isoelectric point of 3-10. All the 13 (100%) NCC seropositive and 15 (60%) out of 25 NCC seronegative samples were reactive with 2D fraction antigens. In the control group, none of the serum was reactive except 2 hydatid samples (92% specificity). The sensitivity and specificity of 2D-PAGE EITB assay were significantly higher than the ELISA which is the routine diagnostic method used in the endemic countries for the serodiagnosis of neurocysticercosis.
由猪带绦虫囊尾蚴引起的脑囊尾蚴病（NCC）在印度儿童中是一个日益重要的健康问题。在单个囊尾蚴肉芽肿病例中，现有的血清学技术的敏感性和特异性较低，而这在印度患者中更为常见，这些患者多为儿童。从 13 例临床和影像学提示性脑囊尾蚴病儿童中采集血清样本，这些儿童经 ELISA 检测呈阳性，25 例临床和影像学提示性脑囊尾蚴病儿童经 ELISA 检测呈阴性，25 例对照受试者。用 2 维凝胶电泳（2D-PAGE）和酶联免疫电转移印迹（EITB）法检测 T. solium 囊尾蚴 10-30 kDa 抗原的血清抗体，对 T. solium 囊尾蚴 10-30 kDa 抗原进行 2 维凝胶电泳（2D-PAGE）分析，结果显示在 10-28 kDa 之间有 31 种蛋白，在 28-30 kDa 之间有无数种蛋白，等电点为 3-10。13 例（100%）脑囊尾蚴病阳性和 25 例（60%）脑囊尾蚴病阴性样本中的 15 例（60%）与 2D 片段抗原反应。在对照组中，除了 2 个包虫病样本外（92%的特异性），没有血清反应。2D-PAGE EITB 检测的敏感性和特异性明显高于 ELISA，ELISA 是流行国家用于神经囊尾蚴病血清学诊断的常规诊断方法。
