Atluri S R V, Singhi P, Khandelwal N, Malla N
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Parasite Immunol. 2009 Mar;31(3):151-5. doi: 10.1111/j.1365-3024.2008.01085.x.
Neurocysticercosis (NCC), caused by the presence of Taenia solium Cysticerci in the Central Nervous System is the most common neurological disease of parasite aetiology. The serodiagnostic methods available at present have variable sensitivity and specificity depending upon the antigen and technique used. The present study was aimed to assess the efficacy of T. solium Cysticerci excretory secretory (ES) and lower molecular mass (LMM) 10-30 kDa antigenic fractions for antibody detection in serum and urine samples by enzyme-linked immunoelectrotransfer blot (EITB) for the diagnosis of NCC. Serum and urine samples were collected from 125 clinically suspected and radiologically proven NCC children (111 patients with single lesion and 14 with multiple lesions) and 125 control subjects. With the use of ES and LMM antigenic fractions, the sensitivity of the EITB assay was 85.6% and 80.8% with serum and 76.8% and 50.4% with urine, respectively. The specificity was 64% and 61.6% with serum and 48% and 33.6% with urine samples, respectively. The study suggests that antibody detection to ES antigen in serum by EITB assay may serve better purpose for the serodiagnosis of human NCC.
神经囊尾蚴病（NCC）是由猪带绦虫囊尾蚴寄生于中枢神经系统引起的，是寄生虫病因所致最常见的神经系统疾病。目前可用的血清学诊断方法，其敏感性和特异性因所用抗原和技术而异。本研究旨在通过酶联免疫电转移印迹法（EITB）评估猪带绦虫囊尾蚴排泄分泌（ES）和低分子量（LMM）10 - 30 kDa抗原组分在血清和尿液样本中检测抗体用于诊断NCC的效果。从125例临床疑似且经影像学证实的NCC儿童（111例单发病变患者和14例多发病变患者）以及125名对照受试者中采集血清和尿液样本。使用ES和LMM抗原组分时，EITB检测法对血清的敏感性分别为85.6%和80.8%，对尿液的敏感性分别为76.8%和50.4%。对血清的特异性分别为64%和61.6%，对尿液样本的特异性分别为48%和33.6%。该研究表明，通过EITB检测法检测血清中针对ES抗原的抗体可能对人类NCC的血清学诊断更有用。
