Department of Environmental Toxicology, Uppsala University, Norbyvägen 18A, SE-75236 Uppsala, Sweden.
Comp Biochem Physiol C Toxicol Pharmacol. 2011 Jun;154(1):42-55. doi: 10.1016/j.cbpc.2011.02.005. Epub 2011 Feb 25.
Cytochrome P450 1 (CYP1) mRNA induction patterns in three-spined stickleback (Gasterosteus aculeatus) were explored for use in environmental monitoring of aryl hydrocarbon receptor (AHR) agonists. The cDNAs of stickleback CYP1A, CYP1B1, CYP1C1, and CYP1C2 were cloned and their basal and induced expression patterns were determined in the brain, gill, liver and kidney. Also, their induction time courses were compared after waterborne exposure to a transient (indigo) or a persistent (3,3',4,4',5-pentacholorbiphenyl PCB 126) AHR agonist. The cloned stickleback CYP1s exhibited a high amino acid sequence identity compared with their zebrafish orthologs and their constitutive tissue distribution patterns largely agreed with those reported in other species. PCB 126 (100 nM) induced different CYP1 expression patterns in the four tissues, suggesting tissue-specific regulation. Both indigo (1 nM) and PCB 126 (10 nM) induced a strong CYP1 expression in gills. However, while PCB 126 gave rise to a high and persistent induction in gills and liver, induction by indigo was transient in both organs. The number of putative dioxin response elements found in each CYP1 gene promoter roughly reflected the induction levels of the genes. The high responsiveness of CYP1A, CYP1B1, and CYP1C1 observed in several organs suggests that three-spined stickleback is suitable for monitoring of pollution with AHR agonists.
研究了三刺鱼（Gasterosteus aculeatus）细胞色素 P450 1（CYP1）mRNA 的诱导模式，以期将其用于芳烃受体（AHR）激动剂的环境监测。克隆了三刺鱼 CYP1A、CYP1B1、CYP1C1 和 CYP1C2 的 cDNA，并在大脑、鳃、肝脏和肾脏中确定了它们的基础表达和诱导表达模式。此外，还比较了它们在暴露于瞬时（靛蓝）或持久（3,3',4,4',5-五氯联苯 PCB 126）AHR 激动剂的水中后诱导的时间过程。克隆的三刺鱼 CYP1 与斑马鱼同源物具有很高的氨基酸序列同一性，其组成型组织分布模式与其他物种的报道基本一致。PCB 126（100 nM）在四种组织中诱导不同的 CYP1 表达模式，表明存在组织特异性调节。靛蓝（1 nM）和 PCB 126（10 nM）都能强烈诱导鳃中的 CYP1 表达。然而，虽然 PCB 126 导致鳃和肝脏中高且持久的诱导，但靛蓝在这两个器官中的诱导是短暂的。每个 CYP1 基因启动子中发现的推定二恶英反应元件的数量大致反映了基因的诱导水平。在几个器官中观察到 CYP1A、CYP1B1 和 CYP1C1 的高反应性表明，三刺鱼适合用于监测 AHR 激动剂的污染。
