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本文引用的文献

1
Biomarkers of organic contamination in the South American fish Poecilia vivipara and Jenynsia multidentata.南美的胎生鱂鱼和多齿詹尼鱼的有机污染生物标志物。
J Toxicol Environ Health A. 2012;75(16-17):1023-34. doi: 10.1080/15287394.2012.697813.
2
Cytochrome P450 1A, 1B, and 1C mRNA induction patterns in three-spined stickleback exposed to a transient and a persistent inducer.三种棘鱼暴露于瞬时和持续诱导剂时细胞色素 P450 1A、1B 和 1C mRNA 的诱导模式。
Comp Biochem Physiol C Toxicol Pharmacol. 2011 Jun;154(1):42-55. doi: 10.1016/j.cbpc.2011.02.005. Epub 2011 Feb 25.
3
New CYP1 genes in the frog Xenopus (Silurana) tropicalis: induction patterns and effects of AHR agonists during development.在热带爪蟾（Silurana）中发现的新 CYP1 基因：发育过程中 AHR 激动剂的诱导模式和影响。
Toxicol Appl Pharmacol. 2011 Jan 15;250(2):170-83. doi: 10.1016/j.taap.2010.10.010. Epub 2010 Oct 18.
4
Induction patterns of new CYP1 genes in environmentally exposed rainbow trout.环境暴露虹鳟鱼中新 CYP1 基因的诱导模式。
Aquat Toxicol. 2010 Jul 15;98(4):311-21. doi: 10.1016/j.aquatox.2010.03.003. Epub 2010 Mar 11.
5
New cytochrome P450 1B1, 1C2 and 1D1 genes in the killifish Fundulus heteroclitus: Basal expression and response of five killifish CYP1s to the AHR agonist PCB126.青鳉鱼（Fundulus heteroclitus）中的新型细胞色素P450 1B1、1C2和1D1基因：五种青鳉鱼细胞色素P450的基础表达及对芳烃受体激动剂多氯联苯126的反应
Aquat Toxicol. 2009 Jul 26;93(4):234-43. doi: 10.1016/j.aquatox.2009.05.008. Epub 2009 May 15.
6
Cytochrome P450 1D1: a novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD.细胞色素P450 1D1：一种与CYP1A相关的新基因，其不会被多氯联苯126或四氯二苯并二恶英转录激活。
Arch Biochem Biophys. 2009 Feb;482(1-2):7-16. doi: 10.1016/j.abb.2008.12.002. Epub 2008 Dec 10.
7
Analyzing real-time PCR data by the comparative C(T) method.通过比较Ct法分析实时荧光定量PCR数据。
Nat Protoc. 2008;3(6):1101-8. doi: 10.1038/nprot.2008.73.
8
Gene structure of the novel cytochrome P4501D1 genes in stickleback (Gasterosteus aculeatus) and medaka (Oryzias latipes).棘鱼（三刺鱼）和青鳉（日本青鳉）中新型细胞色素P4501D1基因的基因结构。
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Cytochrome P450 1 genes in early deuterostomes (tunicates and sea urchins) and vertebrates (chicken and frog): origin and diversification of the CYP1 gene family.早期后口动物（被囊动物和海胆）及脊椎动物（鸡和青蛙）中的细胞色素P450 1基因：CYP1基因家族的起源与多样化
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巴西青鳉鱼（Poecilia vivipara）中细胞色素 P450 1A、1B 和 1C 基因的基础表达和 3-甲基胆蒽诱导表达。

Basal and 3-methylcholanthrene-induced expression of cytochrome P450 1A, 1B and 1C genes in the Brazilian guppy, Poecilia vivipara.

机构信息

Biochemistry Department, Universidade Federal de Santa Catarina, SC, Brazil.

出版信息

Aquat Toxicol. 2012 Nov 15;124-125:106-13. doi: 10.1016/j.aquatox.2012.08.006. Epub 2012 Aug 14.

DOI:10.1016/j.aquatox.2012.08.006
PMID:22940225
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4349553/
Abstract

In fish there are four cytochrome P450 (CYP1) subfamilies: CYP1A, CYP1B, CYP1C, and CYP1D. Here we cloned Poecilia vivipara CYP1A, with an inferred amino acid sequence 91% identical to CYP1A from the killifish Fundulus heteroclitus, another member of the Cypriniformes, and an important model in ecotoxicology. In addition, we examined the expression of CYP1A, CYP1B1, and CYP1C1 by qPCR in liver, gill, and intestine of adult P. vivipara injected with 3-methylcholanthrene (3-MC) or held in clean water (control group) for 24h. All three tissues examined showed basal expression of the three CYP1 genes. CYP1A was most strongly expressed in the liver, while CYP1B1, and CYP1C1 were most strongly expressed in the gill and intestine respectively. 3-MC induced CYP1A, CYP1B1, and CYP1C1 significantly (20-120-fold) in the three organs, consistent with the regulation of CYP1A, CYP1B1 and CYP1C1 via the aryl hydrocarbon receptor. Validation of CYP1 gene biomarkers in fish collected from a contaminated urban mangrove environment was confirmed with significant induction of CYP1A and CYP1C1 in gills (10-15-fold) and CYP1B1 in liver (23-fold), relative to fish from a control site. The responsiveness of these CYP1 genes indicates P. vivipara is suitable as a model for environmental toxicology studies and environmental assessment in Brazil.

摘要

鱼类中存在四个细胞色素 P450（CYP）亚家族：CYP1A、CYP1B、CYP1C 和 CYP1D。在这里，我们克隆了胎生鱂鱼的 CYP1A，其推断的氨基酸序列与另一种脂鲤目鱼类食蚊鱼（Fundulus heteroclitus）的 CYP1A 有 91%的同源性，食蚊鱼是生态毒理学的重要模式生物。此外，我们通过 qPCR 检测了成年胎生鱂鱼在注射 3-甲基胆蒽（3-MC）或置于清洁水中（对照组）24 小时后肝脏、鳃和肠道中 CYP1A、CYP1B1 和 CYP1C1 的表达情况。所有三种组织均显示出三种 CYP1 基因的基础表达。CYP1A 在肝脏中表达最强，而 CYP1B1 和 CYP1C1 在鳃和肠道中表达最强。3-MC 在三种器官中均显著诱导了 CYP1A、CYP1B1 和 CYP1C1（20-120 倍），这与 CYP1A、CYP1B1 和 CYP1C1 通过芳香烃受体的调节一致。通过对取自受污染城市红树林环境的鱼类进行 CYP1 基因生物标志物的验证，发现鳃组织中 CYP1A 和 CYP1C1（10-15 倍）以及肝脏中 CYP1B1（23 倍）的诱导显著，相对于对照点的鱼类。这些 CYP1 基因的反应性表明，胎生鱂鱼适合作为巴西环境毒理学研究和环境评估的模型。