Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE 405 30, Gothenburg, Sweden; Biology Department, MS #32, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.
Biology Department, MS #32, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, USA.
Environ Toxicol Pharmacol. 2021 Apr;83:103580. doi: 10.1016/j.etap.2020.103580. Epub 2021 Jan 8.
Previous reports suggested that non-dioxin-like (NDL) PCB153 effects on cytochrome P450 3A (Cyp3a) expression in Atlantic killifish (Fundulus heteroclitus) gills differed between F0 generation fish from a PCB site (New Bedford Harbor; NBH) and a reference site (Scorton Creek; SC). Here, we examined effects of PCB153, dioxin-like (DL) PCB126, or a mixture of both, on Cyp3a56 mRNA in killifish generations removed from the wild, without environmental PCB exposures. PCB126 effects in liver and gills differed between populations, as expected. Gill Cyp3a56 was not affected by either congener in NBH F2 generation fish, but was induced by PCB153 in SC F1 fish, with females showing a greater response. PCB153 did not affect Cyp3a56 in liver of either population. Results suggest a heritable resistance to NDL-PCBs in killifish from NBH, in addition to that reported for DL PCBs. Induction of Cyp3a56 in gills may be a biomarker of exposure to NDL PCBs in fish populations that are not resistant to PCBs.
先前的报告表明，非二恶英类（NDL）多氯联苯 153（PCB153）对大西洋丽鱼（Fundulus heteroclitus）鳃细胞色素 P4503A（Cyp3a）表达的影响在源自 PCB 污染场地（新贝德福德港；NBH）和参照场地（斯科顿克里克；SC）的 F0 代鱼之间存在差异。在这里，我们研究了 PCB153、二恶英类（DL）多氯联苯 126 或两者混合物对源自野外、未接触环境 PCB 的丽鱼各世代 Cyp3a56mRNA 的影响。如预期的那样，PCB126 在肝脏和鳃中的作用在种群之间存在差异。NBH F2 代鱼的鳃细胞 Cyp3a56 不受任何同系物的影响，但 SC F1 鱼中的 PCB153 诱导其表达，而雌性鱼的反应更大。PCB153 对来自两个种群的肝脏 Cyp3a56 均无影响。结果表明，除了对 DL PCB 的报道外，NBH 的丽鱼还具有对 NDL-PCBs 的遗传抗性。鳃中 Cyp3a56 的诱导可能是对未抵抗 PCB 的鱼类种群中 NDL PCBs 暴露的生物标志物。
