Neiker, Granja Modelo de Arkaute, Vitoria-Gasteiz, Spain.
Fungal Biol. 2011 Mar;115(3):288-95. doi: 10.1016/j.funbio.2010.12.014. Epub 2011 Jan 5.
Pitch canker, caused by Fusarium circinatum, is a destructive disease of Pinus species and has recently been shown to represent a substantial threat to natural and commercial forests in northern Spain. The genetic diversity of F. circinatum in the Basque Country of Spain was assessed by characterising 96 isolates based on vegetative compatibility groups (VCGs), mating type assays, polymorphic DNA-markers and amplified fragment length polymorphism (AFLP) analyses. For this purpose, F. circinatum isolates were collected from diseased Pinus radiata as well as from insects associated with this host. Overall, a low level of diversity was detected in the population. The isolates represented only two VCGs and they were all of the same mating type. AFLP analyses revealed three genotypes and polymorphic DNA-markers specific for F. circinatum showed nine genotypes. The most common genotypes represented 97% of all isolates for AFLP analysis and 68% of isolates for the polymorphic DNA-marker sets. Over all, this indicates that pitch canker in the Basque Country of Spain is caused by a clonally propagating population of F. circinatum, typical of a recently introduced pathogen.
球腔菌溃疡病，由尖孢镰刀菌引起，是松树物种的一种破坏性疾病，最近已被证明对西班牙北部的天然林和商业林构成了重大威胁。通过基于营养体亲和群（VCG）、交配型测定、多态性 DNA 标记和扩增片段长度多态性（AFLP）分析，对西班牙巴斯克地区的尖孢镰刀菌的遗传多样性进行了评估。为此，从患病的辐射松和与该宿主相关的昆虫中收集了尖孢镰刀菌分离物。总体而言，在该种群中检测到的多样性水平较低。分离物仅代表两个 VCG，且均为相同的交配型。AFLP 分析显示出三种基因型，而特异性尖孢镰刀菌的多态性 DNA 标记显示出 9 种基因型。最常见的基因型代表 AFLP 分析中所有分离物的 97%，以及多态性 DNA 标记组中所有分离物的 68%。总的来说，这表明西班牙巴斯克地区的球腔菌溃疡病是由尖孢镰刀菌的无性繁殖种群引起的，这是一种最近引入的病原体的典型特征。
