Zanello Galliano, Meurens François, Berri Mustapha, Chevaleyre Claire, Melo Sandrine, Auclair Eric, Salmon Henri
Institut National de la Recherche Agronomique (INRA), UR1282, Infectiologie Animale et Santé Publique, F-37380 Nouzilly (Tours), Indre et Loire, France.
Vet Immunol Immunopathol. 2011 May 15;141(1-2):133-8. doi: 10.1016/j.vetimm.2011.01.018. Epub 2011 Feb 26.
Probiotic yeasts may provide protection against intestinal inflammation induced by enteric pathogens. In piglets, infection with F4+ enterotoxigenic Escherichia coli (ETEC) leads to inflammation, diarrhea and intestinal damage. In this study, we investigated whether the yeast strains Saccharomyces cerevisiae (Sc, strain CNCM I-3856) and S. cerevisiae variety boulardii (Sb, strain CNCM I-3799) decreased the expression of pro-inflammatory cytokines and chemokines in intestinal epithelial IPI-2I cells cultured with F4+ ETEC. Results showed that viable Sc inhibited the ETEC-induced TNF-α gene expression whereas Sb did not. In contrast, killed Sc failed to inhibit the expression of pro-inflammatory genes. This inhibition was dependent on secreted soluble factors. Sc culture supernatant decreased the TNF-α, IL-1α, IL-6, IL-8, CXCL2 and CCL20 ETEC-induced mRNA. Furthermore, Sc culture supernatant filtrated fraction < 10 kDa displayed the same effects excepted for TNF-α. Thus, our results extended to Sc (strain CNCM I-3856) the inhibitory effects of some probiotic yeast strains onto inflammation.
益生菌酵母可能对肠道病原体引起的肠道炎症具有保护作用。在仔猪中，感染F4 + 产肠毒素大肠杆菌（ETEC）会导致炎症、腹泻和肠道损伤。在本研究中，我们调查了酿酒酵母菌株（Sc，菌株CNCM I - 3856）和酿酒酵母变种布拉迪酵母（Sb，菌株CNCM I - 3799）是否能降低与F4 + ETEC共培养的肠上皮IPI - 2I细胞中促炎细胞因子和趋化因子的表达。结果表明，活的Sc抑制了ETEC诱导的TNF-α基因表达，而Sb则没有。相反，灭活的Sc未能抑制促炎基因的表达。这种抑制作用依赖于分泌的可溶性因子。Sc培养上清液降低了ETEC诱导的TNF-α、IL-1α、IL-6、IL-8、CXCL2和CCL20的mRNA水平。此外，除TNF-α外，过滤后的分子量小于10 kDa的Sc培养上清液组分显示出相同的效果。因此，我们的研究结果将某些益生菌酵母菌株对炎症的抑制作用扩展到了Sc（菌株CNCM I - 3856）。
