Kim Kwangwook, He Yijie, Jinno Cynthia, Kovanda Lauren, Li Xunde, Bravo David, Cox Eric, Liu Yanhong
Department of Animal Science, University of California, Davis, CA, 95616, USA.
School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
J Anim Sci Biotechnol. 2022 Jan 12;13(1):10. doi: 10.1186/s40104-021-00655-2.
There is a great demand for antibiotic alternatives to maintain animal health and productivity. The objective of this experiment was to determine the efficacy of dietary supplementation of a blood group A6 type 1 antigen oligosaccharides-based polymer (Coligo) on growth performance, diarrhea severity, intestinal health, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli (ETEC), when compared with antibiotics.
Pigs in antibiotic carbadox or Coligo treatment groups had greater (P < 0.05) body weight on d 5 or d 11 post-inoculation (PI) than pigs in the control group, respectively. Supplementation of antibiotics or Coligo enhanced (P < 0.05) feed efficiency from d 0 to 5 PI and reduced (P < 0.05) frequency of diarrhea throughout the experiment, compared with pigs in the control group. Supplementation of antibiotics reduced (P < 0.05) fecal β-hemolytic coliforms on d 2, 5, and 8 PI. Pigs in antibiotics or Coligo groups had reduced (P < 0.05) neutrophil counts and serum haptoglobin concentration compared to pigs in the control group on d 2 and 5 PI. Pigs in Coligo had reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 5 and 11 PI, whereas pigs in antibiotics or Coligo groups had reduced (P < 0.05) total coliforms in spleen on d 11 PI compared with pigs in the control group. On d 5 PI, pigs in the Coligo group had greater (P < 0.05) gene expression of ZO1 in jejunal mucosa, but less (P < 0.05) mRNA expression of IL1B, IL6, and TNF in ileal mucosa, in comparison with pigs in the control group. Supplementation of antibiotics enhanced (P < 0.05) the gene expression of OCLN in jejunal mucosa but decreased (P < 0.05) IL1B and IL6 gene expression in ileal mucosa, compared with the control. On d 11 PI, supplementation of antibiotics or Coligo up-regulated (P < 0.05) gene expression of CLDN1 in jejunal mucosa, but Coligo reduced (P < 0.05) IL6 gene expression in ileal mucosa compared to pigs in the control group.
Supplementation of Coligo improved growth performance, alleviated diarrhea severity, and enhanced gut health in weaned pigs infected with ETEC F18 in a manner similar to in-feed antibiotics.
对抗生素替代品的需求很大，以维持动物健康和生产力。本实验的目的是确定日粮中添加基于A6血型1型抗原寡糖的聚合物（Coligo）对实验性感染产肠毒素大肠杆菌（ETEC）的断奶仔猪生长性能、腹泻严重程度、肠道健康和全身免疫的影响，并与抗生素进行比较。
与对照组相比，抗生素卡巴多司或Coligo处理组的仔猪在接种后第5天或第11天体重更大（P<0.05）。与对照组相比，添加抗生素或Coligo提高了接种后0至5天的饲料效率（P<0.05），并降低了整个实验期间的腹泻频率（P<0.05）。添加抗生素降低了接种后第2天、第5天和第8天粪便中的β-溶血大肠杆菌数量（P<0.05）。与对照组相比，抗生素或Coligo组的仔猪在接种后第2天和第5天中性粒细胞计数和血清触珠蛋白浓度降低（P<0.05）。Coligo组的仔猪在接种后第5天和第11天肠系膜淋巴结中的总大肠杆菌数量减少（P<0.05），而抗生素或Coligo组的仔猪在接种后第11天脾脏中的总大肠杆菌数量与对照组相比减少（P<0.05）。接种后第5天，与对照组相比，Coligo组仔猪空肠黏膜中ZO1的基因表达更高（P<0.05），但回肠黏膜中IL1B、IL6和TNF的mRNA表达更低（P<0.05）。与对照组相比，添加抗生素增强了空肠黏膜中OCLN的基因表达（P<0.05），但降低了回肠黏膜中IL1B和IL6的基因表达（P<0.05）。接种后第11天，添加抗生素或Coligo上调了空肠黏膜中CLDN1的基因表达（P<0.05），但与对照组相比，Coligo降低了回肠黏膜中IL6的基因表达（P<0.05）。
添加Coligo可改善感染ETEC F18的断奶仔猪的生长性能，减轻腹泻严重程度，并增强肠道健康，其方式与饲料中添加抗生素相似。
