Lu Guo-hui, Zhang Shi-zhong, Chen Qiang, Wang Xue-feng, Lu Feng-fei, Liu Jian, Li Ming, Li Zhen-yong
Department of Neurosurgery and Institute of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Feb;31(2):262-5.
To investigate the biological features of human decidua basalis-derived mesenchymal stem cells (PDB-MSCs) in vitro and identify their capacity of multilineage differentiation.
PDB-MSCs were harvested from the decidua basalis of term placental by enzymatic digestion and density gradient centrifugation, and the growth characteristics and morphological changes of the MSCs were observed by inverted microscope. The proliferative ability of the cells was assessed by Cell Counting Kit-8. The cell cycle and expressions of the surface markers (CD29, CD44, CD73, CD90, CD34, CD45, and CD14) of the MSCs were identified by flow cytometry. Multilineage differentiation capacity of the cells was tested by inducing their differentiation toward osteoblasts, adipocytes and chondroblasts in vitro.
MSCs isolated from human decidua basalis of term placental exhibited a morphology similar to that of bone marrow-derived MSCs, and grew into colonies in in vitro culture, where the cells proliferated rapidly after passage with a cell doubling time of 2.21∓0.21 days. More than 70% of the cells stayed in the resting stage (G(0)/G(1)) and showed positivity for CD29, CD44, CD73 and CD90, but not for CD14, CD34 or CD45. After induction, the cells showed positive results of alizarin red staining, oil red O staining and Alcian blue staining.
Human decidua basalis contains a rich source of MSCs, which can be easily isolated and cultured without affecting their capacity of multilineage differentiation. The PDB-MSCs may have the potential as a novel source of stem cells.
研究人胎盘基蜕膜间充质干细胞（PDB-MSCs）的体外生物学特性，并鉴定其多向分化能力。
采用酶消化法和密度梯度离心法从足月胎盘的基蜕膜中获取PDB-MSCs，通过倒置显微镜观察MSCs的生长特性和形态变化。采用细胞计数试剂盒-8评估细胞的增殖能力。通过流式细胞术鉴定MSCs的细胞周期及表面标志物（CD29、CD44、CD73、CD90、CD34、CD45和CD14）的表达。通过体外诱导细胞向成骨细胞、脂肪细胞和软骨细胞分化来检测细胞的多向分化能力。
从足月胎盘人基蜕膜分离的MSCs表现出与骨髓来源MSCs相似的形态，在体外培养中形成集落，传代后细胞增殖迅速，细胞倍增时间为2.21±0.21天。超过70%的细胞处于静止期（G(0)/G(1)），CD29、CD44、CD73和CD90呈阳性，但CD14、CD34或CD45呈阴性。诱导后，细胞茜素红染色、油红O染色和阿尔辛蓝染色呈阳性。
人胎盘基蜕膜含有丰富的MSCs来源，易于分离和培养，且不影响其多向分化能力。PDB-MSCs可能具有作为新型干细胞来源的潜力。
