Jakab Csaba, Rusvai Miklós, Gálfi Péter, Halász Judit, Kulka Janina
Department of Pathology and Forensic Veterinary Medicine, Szent István University, István, Budapest, Hungary.
Acta Vet Hung. 2011 Mar;59(1):87-98. doi: 10.1556/AVet.59.2011.1.8.
Claudin-5 is an endothelium-specific tight junction protein. The aim of the present study was to detect the expression pattern of this molecule in intact pancreatic tissues and in well-differentiated and poorly differentiated pancreatic acinar cell carcinomas from dogs by the use of cross-reactive humanised anticlaudin-5 antibody. The necropsy samples taken from dogs included 10 nonneoplastic pancreatic tissues, 10 well-differentiated pancreatic acinar cell carcinomas, 10 poorly differentiated pancreatic acinar cell carcinomas, 5 intrahepatic metastases of well-differentiated and 5 intrahepatic metastases of poorly differentiated acinar cell carcinomas. A strong lateral membrane claudin-5 positivity was detected in exocrine cells in all intact pancreas samples. The endocrine cells of the islets of Langerhans and the epithelial cells of the ducts were negative for claudin-5. The endothelial cells of vessels and lymphatic channels in the stroma of the intact pancreas showed strong membrane positivity for this claudin. All well-differentiated exocrine pancreas carcinomas and all poorly-differentiated pancreatic acinar cell carcinoma samples showed a diffuse loss of claudin-5 expression. The claudin-5-positive peritumoural vessels and lymphatic channels facilitated the detection of vascular invasion of the claudin-5-negative cancer cells. In liver metastasis samples, the pancreatic carcinomas were negative for claudin-5. It seems that the loss of expression of claudin-5 may lead to carcinogenesis in canine exocrine pancreatic cells.
Claudin-5是一种内皮细胞特异性紧密连接蛋白。本研究的目的是通过使用交叉反应性人源化抗Claudin-5抗体，检测该分子在犬完整胰腺组织以及高分化和低分化胰腺腺泡细胞癌中的表达模式。取自犬的尸检样本包括10个非肿瘤性胰腺组织、10个高分化胰腺腺泡细胞癌、10个低分化胰腺腺泡细胞癌、5个高分化腺泡细胞癌的肝内转移灶和5个低分化腺泡细胞癌的肝内转移灶。在所有完整胰腺样本的外分泌细胞中均检测到Claudin-5在侧膜呈强阳性。胰岛的内分泌细胞和导管的上皮细胞Claudin-5呈阴性。完整胰腺间质中血管和淋巴管的内皮细胞对这种Claudin呈强膜阳性。所有高分化外分泌胰腺癌和所有低分化胰腺腺泡细胞癌样本均显示Claudin-5表达弥漫性缺失。Claudin-5阳性的肿瘤周围血管和淋巴管有助于检测Claudin-5阴性癌细胞的血管侵犯。在肝转移样本中，胰腺癌Claudin-5呈阴性。Claudin-5表达缺失似乎可能导致犬外分泌胰腺细胞发生癌变。
