Department of Physiology and Pathology, Dental School of Araraquara, State University of São Paulo, Araraquara, SP, Brazil.
Int J Dent Hyg. 2011 Nov;9(4):279-83. doi: 10.1111/j.1601-5037.2011.00503.x. Epub 2011 Feb 20.
The aim of this study was to investigate the effectiveness of two alternatives methods for the disinfection of oral cleaning devices.
One type of toothbrush and two types of tongue scrapers (steel and plastic) were tested in this study. Sixteen specimens of each group were cut with standardized dimensions, contaminated separately with Candida albicans, Streptococcus mutans and Staphylococcus aureus and incubated for 24 h. After this, oral cleaning devices were washed in saline solution to remove non-adhered cells and divided into two groups (n = 8), one irradiated in microwave and other immersed in 3.78% sodium perborate solution, and evaluated for microbial recovery. The values of cfu of each group of microorganism after disinfection were compared by Kruskal-Wallis and Dunn non-parametric test, considering 95% of confidence.
The toothbrush harboured a significant larger number of viable organisms than the tongue scrapers. The steel tongue scraper was less susceptible to adhesion of the three oral microorganisms. The time required to inactivate all contaminating microorganisms using microwave oven was 1 min and, for the immersion in 3.78% sodium perborate solution, was 2 and 3 h, respectively, for C. albicans and S. mutans/S. aureus.
Microwave irradiation proved to be an effective alternative method to the disinfection of tongue cleaners and toothbrushes.
本研究旨在探讨两种替代口腔清洁器具消毒方法的有效性。
本研究测试了一种牙刷和两种类型的舌刮板（钢和塑料）。每组 16 个标本均按标准尺寸切割，分别用白色念珠菌、变形链球菌和金黄色葡萄球菌污染，并孵育 24 小时。然后，用生理盐水清洗口腔清洁器具以去除非黏附细胞，并将其分为两组（n = 8），一组用微波照射，另一组浸入 3.78%过硼酸钠溶液中，并评估微生物回收情况。通过 Kruskal-Wallis 和 Dunn 非参数检验比较每组微生物的 cfu 值，置信度为 95%。
牙刷比舌刮板携带更多的活菌。钢舌刮板对三种口腔微生物的黏附性较低。使用微波炉灭活所有污染微生物所需的时间为 1 分钟，而在 3.78%过硼酸钠溶液中分别需要 2 小时和 3 小时才能使白色念珠菌和变形链球菌/金黄色葡萄球菌失活。
微波辐射被证明是一种有效的替代方法，可用于消毒舌刮板和牙刷。
