Faculdade de Odontologia de São José dos Campos, Univ Estadual Paulista, São José dos Campos, SP, Brazil.
Braz Oral Res. 2010 Jan-Mar;24(1):28-33. doi: 10.1590/s1806-83242010000100005.
The aim of this study was to evaluate alternative methods for the disinfection of toothbrushes considering that most of the previously proposed methods are expensive and cannot be easily implemented. Two-hundred toothbrushes with standardized dimensions and bristles were included in the study. The toothbrushes were divided into 20 experimental groups (n = 10), according to microorganism considered and chemical agent used. The toothbrushes were contaminated in vitro by standardized suspensions of Streptococcus mutans, Streptococcus pyogenes, Staphylococcus aureus or Candida albicans. The following disinfectants were tested: 0.12% chlorhexidine digluconate, 50% white vinegar, a triclosan-containing dentifrice solution, and a perborate-based tablet solution. The disinfection method was immersion in the disinfectant for 10 min. After the disinfection procedure, the number of remaining microbial cells was evaluated. The values of cfu/toothbrush of each group of microorganism after disinfection were compared by Kruskal-Wallis ANOVA and Dunn's test for multiple comparisons (5%). The chlorhexidine digluconate solution was the most effective disinfectant. The triclosan-based dentifrice solution promoted a significant reduction of all microorganisms' counts in relation to the control group. As to the disinfection with 50% vinegar, a significant reduction was observed for all the microorganisms, except for C. albicans. The sodium perborate solution was the less effective against the tested microorganisms. Solutions based on triclosan-containing dentifrice may be considered effective, nontoxic, cost-effective, and an easily applicable alternative for the disinfection of toothbrushes. The vinegar solution reduced the presence of S. aureus, S. mutans and S. pyogenes on toothbrushes.
本研究旨在评估替代牙刷消毒的方法，因为之前提出的大多数方法都很昂贵，而且不容易实施。本研究纳入了 200 把具有标准化尺寸和刷毛的牙刷。根据所考虑的微生物和所用化学剂，将牙刷分为 20 个实验组（n = 10）。将牙刷通过标准化的变异链球菌、酿脓链球菌、金黄色葡萄球菌或白色念珠菌悬液进行体外污染。测试了以下消毒剂：0.12%葡萄糖酸氯己定、50%白醋、含三氯生的牙膏溶液和过硼酸钠片剂溶液。消毒方法为将牙刷浸泡在消毒剂中 10 分钟。消毒程序后，评估剩余微生物细胞的数量。通过 Kruskal-Wallis ANOVA 和 Dunn 检验进行多重比较（5%）比较每个微生物组的菌落形成单位/牙刷值。葡萄糖酸氯己定溶液是最有效的消毒剂。含三氯生的牙膏溶液与对照组相比，显著降低了所有微生物的计数。对于 50%的醋溶液，除白色念珠菌外，所有微生物的数量都有显著减少。过硼酸钠溶液对测试的微生物的消毒效果最差。含三氯生的牙膏溶液可能被认为是一种有效、无毒、经济高效且易于应用的替代牙刷消毒方法。醋溶液减少了牙刷上金黄色葡萄球菌、变异链球菌和酿脓链球菌的存在。
