牛病毒性腹泻病毒感染后羊驼和牛细胞中的病毒复制和 IFN 反应比较。
Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection.
机构信息
School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, East Campus Loop and Fair Street, Lincoln, NE 68583–0905, USA.
出版信息
Virology. 2011 Apr 25;413(1):111-7. doi: 10.1016/j.virol.2011.02.006. Epub 2011 Feb 26.
Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissiveness, viral replication and interferon (IFN) synthesis was evaluated. BVDV replicated 3-4 logs lower in AT cells with diminished antigen deposition compared to BT cells. BVDV infection inhibited IFN response in both AT and BT cells. Compared to BT cells, BVDV-infected AT cells had a 2-5 fold increase in IFN synthesis following dsRNA stimulation. The greater IFN response of AT cells compared to BT cells following poly I:C stimulation with or without ncp BVDV infection, may be the basis for the decreased BVDV permissiveness of AT cells and may contribute to the clinical differences following BVDV infection of alpacas and cattle.
与牛相比，羊驼感染牛病毒性腹泻病毒（BVDV）后疾病的严重程度降低。我们假设羊驼和牛细胞对 BVDV 感染的易感性和反应存在差异。为了研究羊驼睾丸（AT）和牛鼻甲（BT）细胞对 BVDV 感染的易感性，我们评估了病毒复制和干扰素（IFN）合成。与 BT 细胞相比，BVDV 在 AT 细胞中的复制少了 3-4 个对数级，抗原沉积也减少。BVDV 感染抑制了 AT 和 BT 细胞中的 IFN 反应。与 BT 细胞相比，BVDV 感染的 AT 细胞在 dsRNA 刺激后 IFN 的合成增加了 2-5 倍。与 BT 细胞相比，AT 细胞在 poly I:C 刺激下，无论是有无 ncp BVDV 感染，IFN 的反应都会增加，这可能是 AT 细胞对 BVDV 易感性降低的基础，也可能是羊驼和牛感染 BVDV 后临床差异的基础。
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