Life Sciences, Anglia Ruskin University, East Road, Cambridge, CB1 1PT, United Kingdom.
Environ Pollut. 2011 May;159(5):1446-50. doi: 10.1016/j.envpol.2011.01.031. Epub 2011 Feb 26.
Exposure to the nonsteroidal anti-inflammatory drug (NSAID) diclofenac resulted in the near extinction of three species of Gyps vultures on the Indian subcontinent. Other NSAIDs present in the environment, including flunixin, may pose a similar risk. In the course of a study to determine the feasibility of detecting NSAIDs in keratinous matrices (i.e., hair, nails and feathers) using GC-MS, wool opportunistically collected from a sheep treated with flunixin was analysed for residues. Flunixin was detected qualitatively in external wool wash and extract samples. While residues of veterinary agents and pesticides have previously been found in sheep's wool, our preliminary investigation provides the first instance of an NSAID being detected in this matrix. Here we provide the sample preparation methods and GC-MS parameters used to enable further refinement as part of ongoing conservation and consumer quality control measures.
暴露于非甾体抗炎药（NSAID）双氯芬酸导致印度次大陆上的三种食腐秃鹫接近灭绝。环境中存在的其他 NSAIDs，包括氟尼辛，可能构成类似的风险。在一项使用 GC-MS 检测角蛋白基质（即毛发、指甲和羽毛）中 NSAIDs 的可行性研究中，对从用氟尼辛治疗的绵羊中偶然收集的羊毛进行了残留分析。在外部羊毛洗涤和提取物样品中定性检测到氟尼辛。虽然此前在绵羊的羊毛中发现了兽医制剂和杀虫剂的残留，但我们的初步调查提供了 NSAID 首次在该基质中被检测到的实例。在这里，我们提供了用于进一步改进的样品制备方法和 GC-MS 参数，作为正在进行的保护和消费者质量控制措施的一部分。
