Dipartimento di Biologia Strutturale e Funzionale, Università di Napoli Federico II, Napoli, Italy.
Plant Physiol Biochem. 2011 Apr;49(4):435-40. doi: 10.1016/j.plaphy.2011.02.004. Epub 2011 Feb 18.
In plants, the decline of poly(ADP-ribosyl)ation activity is involved in energy homeostasis and stress tolerance. By reducing stress-induced poly(ADP-ribosyl)ation activity, NAD(+) breakdown is inhibited preventing high energy consumption. Under these conditions, plants preserve their energy homeostasis without an overactivation of mitochondrial respiration, thus avoiding the production of reactive oxygen species. Therefore, plants with lowered poly(ADP-ribosyl)ation activity appear tolerant to multiple stresses. In this study, the evergreen species Cistus incanus L. was used as a model because of its capacity to overcome successfully the environmental constraints of the Mediterranean climate. The aim of the present work was to characterize and assess the role of poly(ADP-ribosyl)ation in C. incanus plants kept under different temperature in greenhouse (GH), outdoor during winter (WO) and outdoor during spring (SO). Data showed that in C. incanus polyADPribose metabolism occurs. The enzyme responsible for poly(ADP-ribose) chains synthesis is a poly(ADP-ribose)polymerase of about 80 kDa, lacking "zinc finger" N-terminal domain and able to automodify. The lowest PARP activity, as well as the lowest quantum yield of PSII linear electron transport (Φ(PSII)) and photochemical quenching (q(P)), was found in WO plants. Instead, in SO plants the recovery of photochemical activity associated to a poly(ADP-ribose)polymerase activity increase of about 50%, as compared to GH plants, was observed. Taking into account both biochemical and eco-physiological responses, a possible explanation for the poly(ADP-ribosyl)ation deficiency in WO plants has been hypothesized.
在植物中，多聚（ADP-核糖）化活性的下降与能量平衡和应激耐受有关。通过降低应激诱导的多聚（ADP-核糖）化活性，抑制 NAD（+）的分解，防止能量消耗过高。在这些条件下，植物保持其能量平衡，而不会过度激活线粒体呼吸，从而避免产生活性氧。因此，多聚（ADP-核糖）化活性降低的植物对多种应激具有耐受性。在这项研究中，常绿物种 Cistus incanus L. 被用作模型，因为它有能力成功克服地中海气候的环境限制。本研究的目的是表征和评估多聚（ADP-核糖）化在 C. incanus 植物中的作用，这些植物分别在温室（GH）、冬季室外（WO）和春季室外（SO）条件下生长。研究数据表明，C. incanus 中存在多 ADP 核糖代谢。负责多聚（ADP-核糖）链合成的酶是一种约 80 kDa 的多聚（ADP-核糖）聚合酶，缺乏“锌指”N 端结构域，能够自动修饰。WO 植物中的 PARP 活性最低，以及 PSII 线性电子传递（Φ（PSII））和光化学猝灭（q（P））的量子产率最低。相比之下，在 SO 植物中，与 GH 植物相比，观察到光化学活性的恢复伴随着多聚（ADP-核糖）聚合酶活性增加约 50%。考虑到生化和生态生理反应，WO 植物中多聚（ADP-核糖）化不足的可能原因被假设。
