Chazotte Brad
CSH Protoc. 2008 Feb 1;2008:pdb.prot4928. doi: 10.1101/pdb.prot4928.
INTRODUCTIONThe eukaryotic cell has evolved to compartmentalize its functions and transport various metabolites among cellular compartments. Therefore, in cell biology, the study of organization and structure/function relationships is of great importance. Integral membrane protein receptors can be studied using specifically fluoresceinated but still functional hormones or toxins that bind to specific receptors. α-bungarotoxin binds with high affinity to the α-subunit of the nicotinic acetylcholine receptor of the neuromuscular junction. In this article, acetylcholine receptors are labeled with α-bungarotoxin conjugated to rhodamine.
引言
真核细胞已经进化出将其功能区室化并在细胞区室之间运输各种代谢物的能力。因此，在细胞生物学中，研究组织以及结构/功能关系非常重要。可以使用与特定受体结合的经过特殊荧光标记但仍具有功能的激素或毒素来研究整合膜蛋白受体。α-银环蛇毒素与神经肌肉接头处烟碱型乙酰胆碱受体的α亚基具有高亲和力结合。在本文中，乙酰胆碱受体用与罗丹明偶联的α-银环蛇毒素进行标记。
