Hodel Corinne, Neuhauss Stephan C F
Institute of Zoology, Neurobiology, University of Zurich, 8057 Zurich, Switzerland.
CSH Protoc. 2008 Mar 1;2008:pdb.prot4961. doi: 10.1101/pdb.prot4961.
INTRODUCTIONLarge movements in the visual field trigger stereotypic eye movements in vertebrates. This optokinetic response (OKR) is robust in zebrafish (Danio rerio) larvae older than 4 days post-fertilization (dpf), and is hence ideally suited for use in the evaluation of visual performance after genetic manipulations. This protocol describes a simple method by which a computer-generated visual stimulus is projected onto a screen and watched by immobilized larvae. The resulting eye movements are recorded and evaluated automatically, using a digital video camera and appropriate computer software. Depending on the sophistication of the software used, the evaluation can be performed in real time during the recording session, or by analyzing the recorded movie files off-line.
引言
视野中的大幅度运动可触发脊椎动物的刻板眼球运动。这种视动反应（OKR）在受精后4天（dpf）以上的斑马鱼（Danio rerio）幼体中很强烈，因此非常适合用于评估基因操作后的视觉性能。本方案描述了一种简单的方法，通过该方法将计算机生成的视觉刺激投射到屏幕上，并由固定的幼体观看。使用数字摄像机和适当的计算机软件自动记录并评估由此产生的眼球运动。根据所使用软件的复杂程度，评估可以在记录过程中实时进行，也可以通过离线分析记录的电影文件来进行。
