Brockerhoff Susan E
Department of Biochemistry, Box 357350, University of Washington, Seattle, Washington 98195, USA.
Nat Protoc. 2006;1(5):2448-51. doi: 10.1038/nprot.2006.255.
Our laboratory screens for visual mutants by examining larval eye movements in response to rotating illuminated stripes. This behavior, which is termed an optokinetic response (OKR), is a reflex that appears in zebrafish at the same time as the development of the visual system. The OKR can be accurately measured by 4 d post-fertilization, which is the age when larvae begin foraging for food. The OKR requires approximately 1 min per larva analyzed. After identifying fish with defective eye movements, we conduct secondary screens (such as histological analysis and electroretinography) to identify the subset of fish with disruptions in the function of the outer retina. This paper describes our protocol for the OKR. Our setup is simple to construct and the materials needed are inexpensive. This makes our system especially useful for new undergraduate and graduate students, as well as introductory science lecturers.
我们的实验室通过检查幼虫对旋转照明条纹的眼动来筛选视觉突变体。这种行为被称为视动反应（OKR），是一种在斑马鱼视觉系统发育同时出现的反射。受精后4天即可准确测量OKR，此时幼虫开始觅食。分析每条幼虫的OKR大约需要1分钟。在识别出眼动有缺陷的鱼后，我们进行二次筛选（如组织学分析和视网膜电图），以确定外视网膜功能受到破坏的鱼的子集。本文描述了我们的OKR实验方案。我们的装置构建简单，所需材料价格低廉。这使得我们的系统对新的本科生和研究生以及基础科学讲师特别有用。
