用于单纳米精度荧光成像（FIONA）的数据分析方法

Data Analysis Methods for Fluorescence Imaging with One-Nanometer Accuracy (FIONA).

作者信息

Selvin Paul R, Lougheed Tyler, Tonks Hoffman Melinda, Park Hyokeun, Balci Hamza, Blehm Benjamin H, Toprak Erdal

机构信息

Biophysics Center and Physics Department, University of Illinois, Urbana, IL, 61801, USA.

出版信息

CSH Protoc. 2007 Oct 1;2007:pdb.ip46. doi: 10.1101/pdb.ip46.

DOI:10.1101/pdb.ip46

PMID:21356943

Abstract

INTRODUCTIONFIONA, short for fluorescence imaging with one-nanometer accuracy, is a simple method for achieving localization of single (or single groups of) fluorophores with nanometer accuracy in the xy plane. Data analysis of a FIONA experiment requires the use of several software programs. Data must be acquired and exported into a proper format before analysis can take place. This article describes various options for data analysis.

摘要

引言

FIONA是“具有一纳米精度的荧光成像”的缩写，是一种在xy平面内以纳米精度实现单个（或单组）荧光团定位的简单方法。FIONA实验的数据分析需要使用多个软件程序。在进行分析之前，必须采集数据并将其导出为合适的格式。本文介绍了数据分析的各种选项。